Role of oxidative stress in osteoblasts exposed to sodium fluoride

Biological Trace Element Research
Hui XuGuang-Sheng Li

Abstract

We investigated the relationship between oxidative stress and osteoblasts viability in osteoblasts exposed to various concentrations of fluoride in this study. Primary calvarial osteoblasts from neonatal Kunming mice were cultured and subcultured to the third generation. Osteoblasts were incubated with sodium fluoride (0, 0.5, 1, 2, 4, 8, 12, and 20 mgF(-)/L) for 24, 48, and 72 h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis showed cell viability significantly increased after osteoblasts exposed to low concentrations of fluoride (0.5 to approximately 2 mgF(-)/L) for 24 to approximately 72 h. Oxidative stress analysis showed that low concentration of fluoride excited lipid peroxidation in osteoblasts and increased activity of antioxidant enzymes in varying degrees. We demonstrated that changes of osteoblasts viability of the low-dose fluoride groups are different from those of high-dose fluoride groups; however, both low and high doses of fluoride caused active state of oxidative stress in osteoblasts, which suggesting that oxidative stress may be excited by the active osteoblasts viability induced by a low dose of fluoride.

References

Jan 1, 1978·Methods in Enzymology·J D CrapoI Fridovich
Aug 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D ModrowskiP J Marie
Oct 1, 1990·Calcified Tissue International·J B Kopp, P G Robey
Nov 6, 1984·Biochimica Et Biophysica Acta·J K HeathJ J Reynolds
Jan 1, 1984·Methods in Enzymology·H Aebi
Jan 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D BurgenerJ Caverzasio
Aug 15, 1994·Biochemical and Biophysical Research Communications·Z Q ZhuJ C Shen
Jan 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P ChavassieuxP J Meunier
Jan 1, 1997·Annual Review of Immunology·H NakamuraJ Yodoi

❮ Previous
Next ❯

Citations

Oct 26, 2010·Biological Trace Element Research·Hui LiuGuang-sheng Li
Sep 21, 2013·Toxicology Letters·Ekambaram PerumalLakshmikanthan Panneerselvam
Apr 15, 2014·International Journal of Nanomedicine·Iwona Inkielewicz-StepniakMarek W Radomski
Jun 6, 2013·Oxidative Medicine and Cellular Longevity·Paula Mochidome YamagutiJosé Nicolau
Jan 24, 2009·Journal of Biomedical Materials Research. Part a·Ana L Di VirgilioMonica Fernández Lorenzo de Mele
Oct 1, 2014·Journal of Toxicology and Environmental Health. Part a·Manivannan JothiramajayamAnita Mukherjee
May 10, 2011·Odontology·Hubertine M E WillemsJenneke Klein-Nulend

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.