Role of p190RhoGAP in beta 2 integrin regulation of RhoA in human neutrophils

The Journal of Immunology : Official Journal of the American Association of Immunologists
K DibT Andersson

Abstract

We found that engagement of beta(2) integrins on human neutrophils induced activation of RhoA, as indicated by the increased ratio of GTP:GTP + GDP recovered on RhoA and translocation of RhoA to a membrane fraction. The clustering of beta(2) integrins also induced a time-dependent increase in GDP bound to RhoA, which correlated with beta(2) integrin-induced activation of p190RHOGAP: The activation of p190RhoGAP was completely blocked by [4-amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine] (PP1), a selective inhibitor of Src family tyrosine kinases. However, clustering of beta(2) integrins did not increase the basal tyrosine phosphorylation of p190RhoGAP, nor did it affect the amount of p120RasGAP bound to p190RHOGAP: Instead, the beta(2) integrin-induced activation of p190RhoGAP was accompanied by increased tyrosine phosphorylation of a p190RhoGAP-associated protein, p120RasGAP, and accumulation of both p120RasGAP and p190RhoGAP in a membrane fraction. PP1 blocked the beta(2) integrin-induced phosphorylation of p120RasGAP, as well as the translocation of p190RhoGAP and p120RasGAP, but it did not affect the accumulation of RhoA in the membrane fraction. In agreement with the mentioned findings, PP1 also increased th...Continue Reading

References

Mar 1, 1990·Scandinavian Journal of Immunology·O W Bjerrum, N Borregaard
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J B GibbsE M Scolnick
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Dec 16, 1993·Nature·M S Boguski, F McCormick
Sep 1, 1993·The Journal of Clinical Investigation·H SengeløvN Borregaard
Apr 26, 1996·The Journal of Biological Chemistry·B SchiefferK E Bernstein
Apr 15, 1997·European Journal of Biochemistry·C G GahmbergP Kotovuori
Feb 7, 1998·Science·A Hall
Jul 29, 1998·The Journal of Cell Biology·E A ClarkR O Hynes

❮ Previous
Next ❯

Citations

Jan 17, 2004·The Journal of Biological Chemistry·Guoyan WangFrank Beier
Jul 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zoltán JakusAttila Mócsai
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yukihiko SugimotoAtsushi Ichikawa
Jun 1, 2006·Acta Physiologica·C Stock, A Schwab
Aug 3, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tamás NémethAttila Mócsai
Jul 5, 2018·European Journal of Clinical Investigation·Roland Csépányi-KömiErzsébet Ligeti
Apr 5, 2003·The Journal of Biological Chemistry·Karim DibTommy Andersson
Sep 10, 2003·Journal of Leukocyte Biology·Maria ForsbergEva Särndahl
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria WeineisenTommy Andersson
Mar 23, 2007·American Journal of Physiology. Renal Physiology·Hui ZhangTomoko Takano
Sep 16, 2009·Blood·Kathleen SzczurMarie-Dominique Filippi
Feb 3, 2012·Physiological Reviews·Erzsébet LigetiKlaus Scheffzek
Sep 18, 2002·Immunological Reviews·Klaus Ley
Feb 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lingling CuiXianlu Zeng
Aug 30, 2003·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Joanna H WebbBjörn Dahlbäck
May 29, 2003·British Journal of Pharmacology·Lara PizurkiAndreas Papapetropoulos
Mar 29, 2006·The Journal of Cell Biology·Agnès WiedemannEmmanuelle Caron
May 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ravi K DeeviKarim Dib
May 3, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carlos Ocaña-MorgnerRolf Jessberger
Apr 30, 2005·Journal of Cell Science·Fiona J PixleyB Hilda Ye
Mar 23, 2007·Journal of Cell Science·Rachel J Buchsbaum
Sep 7, 2001·Current Opinion in Cell Biology·R A Worthylake, K Burridge
Jul 4, 2021·The International Journal of Biochemistry & Cell Biology·Ilie Fadzilah Hashim, Ana Masara Ahmad Mokhtar
Sep 16, 2008·Biochemical and Biophysical Research Communications·Karim DibTommy Andersson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.