Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation

PloS One
Anael Viana Pinto AlbertoLuiz Anastácio Alves

Abstract

ATP and other nucleotides are released from cells through regulated pathways or following the loss of plasma membrane integrity. Once outside the cell, these compounds can activate P2 receptors: P2X ionotropic receptors and G protein-coupled P2Y receptors. Eosinophils represent major effector cells in the allergic inflammatory response and they are, in fact, associated with several physiological and pathological processes. Here we investigate the expression of P2 receptors and roles of those receptors in murine eosinophils. In this context, our first step was to investigate the expression and functionality of the P2X receptors by patch clamping, our results showed a potency ranking order of ATP>ATPγS> 2meSATP> ADP> αβmeATP> βγmeATP>BzATP> UTP> UDP>cAMP. This data suggest the presence of P2X1, P2X2 and P2X7. Next we evaluate by microfluorimetry the expression of P2Y receptors, our results based in the ranking order of potency (UTP>ATPγS> ATP > UDP> ADP >2meSATP > αβmeATP) suggests the presence of P2Y2, P2Y4, P2Y6 and P2Y11. Moreover, we confirmed our findings by immunofluorescence assays. We also did chemotaxis assays to verify whether nucleotides could induce migration. After 1 or 2 hours of incubation, ATP increased migration ...Continue Reading

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Citations

Jan 25, 2019·Annual Review of Immunology·Joel LindenGerhard Dahl
May 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adam WrightCatherine Vial
Oct 11, 2017·Frontiers in Pharmacology·Geoffrey Burnstock
Feb 15, 2019·Current Drug Targets·Natiele C da Silva FerreiraRômulo J Soares-Bezerra
Aug 1, 2020·Frontiers in Immunology·Davide FerrariMarco Idzko
Aug 1, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Haruhisa NishiEdward S Schulman
Nov 30, 2021·IScience·Ross LuuEliseo Eugenin

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Methods Mentioned

BETA
lavage
FCS

Software Mentioned

Axoscope
Image pro

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