Role of p38, ERK1/2, focal adhesion kinase, RhoA/ROCK and cytoskeleton in the adipogenesis of human mesenchymal stem cells

Journal of Bioscience and Bioengineering
Baiyao XuGuanbin Song

Abstract

Adipogenesis is important to health and is thought occurring in the two stages of mesenchymal stem cell commitment to a preadipocyte fate and terminal differentiation of the preadipocyte. However, the mechanism of adipogenesis is still not clear. In this study, the roles of p38, extracellular regulated protein kinases 1/2 (ERK1/2), focal adhesion kinase (FAK), RhoA/ROCK, and cytoskeleton in both of the two stages of adipogenesis were assayed. Our results showed that the treatments of SB203580 (the inhibitor of p38) and U0126 (the inhibitor of ERK1/2) suppressed the adipogenesis induced by differentiation medium, and the treatments of PF573228 (a specific inhibitor of FAK), Y27632 (a specific inhibitor of RhoA/ROCK) and cytochalasin D (an inhibitor of cytoskeletal organization) promoted the adipogenesis. The treatments of SB203580 and U0126 significantly inhibited the adipogenic differentiation of hMSCs cultured in differentiation medium in the presence of PF573228, Y27632 or cytochalasin D. Moreover, the treatments of PF573228, Y27632 and cytochalasin D promoted p38 and ERK1/2 phosphorylations, and the treatments of U0126 and SB203580 decreased p38 and ERK1/2 phosphorylations, respectively. These results demonstrated that p38 a...Continue Reading

References

Nov 21, 1998·The Journal of Biological Chemistry·J A EngelmanP E Scherer
Jan 21, 2001·The Journal of Biological Chemistry·S W KimH S Ro
Oct 19, 2001·Biochemical and Biophysical Research Communications·S TsutsumiY Kato
May 9, 2002·Molecular and Cellular Biology·Teddy T C YangChi-Wing Chow
Nov 22, 2002·American Journal of Respiratory Cell and Molecular Biology·Annette B RiceJames C Bonner
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
Mar 1, 2005·Biochimie·F BostB Binétruy
Jun 1, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Adriana S TorsoniKleber G Franchini
Sep 15, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Valerie E MeyersJay M McDonald
Jan 31, 2006·Diabetes·Myriam AouadiFrédéric Bost
Nov 24, 2006·Investigative Ophthalmology & Visual Science·Zheng WangPeter S Reinach
Apr 20, 2007·Biochemical and Biophysical Research Communications·Jing-Jing Li, Dong Xie
Aug 8, 2007·The Journal of Biological Chemistry·Michio NoguchiKazuwa Nakao
Oct 25, 2007·Cell·Stephane GestaC Ronald Kahn
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Matthew D TreiserPrabhas V Moghe
Feb 12, 2010·Stem Cell Reviews and Reports·Tianshu FengMichal Opas
Feb 5, 2011·Clinical and Experimental Pharmacology & Physiology·Hong-Yan LingDuan-Fang Liao
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Marie Cargnello, Philippe P Roux
Sep 29, 2011·Nature Reviews. Molecular Cell Biology·Ana G Cristancho, Mitchell A Lazar
Nov 10, 2011·Biophysical Journal·Ben FabryWolfgang H Goldmann
Mar 13, 2012·Annual Review of Biophysics·Yubing SunJianping Fu
Apr 3, 2012·Annual Review of Biochemistry·Qi Qun Tang, M Daniel Lane
May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Rafael D González-CruzEric M Darling
Jul 31, 2012·Molecular and Cellular Endocrinology·Aung ThanPeng Chen

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Citations

Oct 16, 2015·Molecular Medicine Reports·Changan PeiShuguang Zhang
Dec 24, 2014·Biochimica Et Biophysica Acta·Changhong KeJiye Cai
Feb 7, 2017·Nature Communications·Cynthia T LukMinna Woo
Feb 10, 2017·Stem Cells and Development·Kan YinRobert Chunhua Zhao
Mar 14, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cui ZhangJinfu Wang
Jan 23, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xin ZhangJingdong Yin
Dec 10, 2015·Experimental Biology and Medicine·Sylvia P PoulosGary J Hausman
Jul 18, 2020·Stem Cell Research & Therapy·Asmat Ullah KhanJingxing Dai
Oct 18, 2020·Nature Reviews. Endocrinology·Ivana NikolicGuadalupe Sabio
Dec 19, 2017·Trends in Biochemical Sciences·Wolfgang DubielMichael Naumann
Apr 17, 2021·Cellular & Molecular Biology Letters·Bing SunJingxing Dai
Jun 13, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Baiyao XuYasuyuki Morita

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