Role of p53 in sensing oxidative DNA damage in response to reactive oxygen species-generating agents

Cancer Research
Geetha Achanta, Peng Huang

Abstract

The tumor suppressor p53 plays an important role in the regulation of cellular response to DNA damage. Recent studies suggest that p53 is able to bind DNA with certain structural alterations in a sequence-independent manner and to interact with several molecules involved in DNA repair. This study was undertaken to test the hypothesis that p53 may participate in sensing oxidative DNA damage, the most frequently occurring spontaneous DNA lesion, and modulate its repair by the base excision repair (BER) machinery. Using synthetic DNA containing 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxoG), we showed that p53 was pulled down together with two BER proteins, human 8-oxoguanine glycosylase (hOGG1) and AP endonuclease (APE). Functional analysis showed that p53 significantly enhanced the sequential activities of hOGG1 and APE in excising the 8-oxoG nucleotide from DNA in vitro. The ability of p53 to enhance the removal of oxidized DNA bases was further demonstrated in vivo using a pair of p53 isogenic lines. HCT116 p53+/+ cells exhibit a more rapid removal of 8-oxoG from DNA than p53-/- cells exposed to the same levels of reactive oxygen species (ROS) stress. Together, these results suggest that p53 participates in sensing oxidative DNA...Continue Reading

References

Jun 1, 1995·Free Radical Biology & Medicine·A P Breen, J A Murphy
Jun 1, 1995·Nature Genetics·X W WangB G Taffe
Sep 2, 1993·Nature·A DuttaE Winchester
Mar 14, 1997·The Journal of Biological Chemistry·S LeeJ Griffith
Feb 4, 1998·Analytical Biochemistry·L StruthersS Thomas
Jul 15, 1998·Critical Reviews in Clinical Laboratory Sciences·H R GriffithsJ Lunec
Mar 5, 1999·Cellular and Molecular Life Sciences : CMLS·R R Wallace-Brodeur, S W Lowe
Oct 3, 1999·The Journal of Biological Chemistry·G BlanderM Oren
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·D R DuckettP Modrich
Dec 22, 1999·Biochemical Pharmacology·C MéplanP Hainaut
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·J M Matés, F M Sánchez-Jiménez
Mar 4, 2000·Nature Structural Biology·S H Wilson, T A Kunkel
Apr 25, 2000·Archives of Biochemistry and Biophysics·S Boiteux, J P Radicella
Jul 20, 2000·Molecular Carcinogenesis·D L CarlisleS R Patierno
Sep 26, 2000·Molecular Genetics and Metabolism·L L Mantell, P J Lee
Dec 1, 2000·Nature·B VogelsteinA J Levine
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·S X LiuT K Hei
Feb 17, 2001·The EMBO Journal·J ZhouC Prives
Dec 18, 2001·Environmental and Molecular Mutagenesis·S MitraT K Hazra
Dec 19, 2001·British Journal of Cancer·E Bálint E, K H Vousden
Oct 3, 2002·Biochemical and Biophysical Research Communications·Nan MeiHiroshi Kasai
Nov 1, 2002·The Journal of Biological Chemistry·Jakub GolabMarek Jakobisiak
Dec 17, 2002·Oncogene·Laurent GrosJacques Laval
May 1, 2004·Trends in Pharmacological Sciences·Lorne J HofsethCurtis C Harris

❮ Previous
Next ❯

Citations

Apr 24, 2013·Chemical Research in Toxicology·Mathias KönczölVolker Mersch-Sundermann
Mar 22, 2008·Cell Death and Differentiation·R U JänickeK Schulze-Osthoff
Nov 16, 2005·Nature Medicine·Anna A SablinaPeter M Chumakov
May 30, 2009·Nature Reviews. Drug Discovery·Dunyaporn TrachoothamPeng Huang
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Sagar Sengupta, Curtis C Harris
Jun 28, 2012·The Journal of Biological Chemistry·Aya MasaokaSamuel H Wilson
May 19, 2007·Antioxidants & Redox Signaling·Ramaneeya NithipongvanitchTerry D Oberley
Oct 6, 2010·Antioxidants & Redox Signaling·Cory U LagoPaul M Hwang
Jun 30, 2012·DNA and Cell Biology·Anna WalczakIreneusz Majsterek
Sep 24, 2005·Journal of Medicinal Food·Jeongmin LeeKun-Young Park
Oct 7, 2008·Mutagenesis·Shaqil N Kassam, Andrew J Rainbow
Aug 25, 2012·Journal of Toxicology·Damian G DeavallRuth Roberts
Oct 7, 2011·Journal of Signal Transduction·Hang CuiHong Zhang
Dec 29, 2006·BMC Cancer·Simon G NyagaMichele K Evans
May 15, 2009·Reproductive Biology and Endocrinology : RB&E·Oleg M AlekseevMichael G O'Rand
Jun 29, 2013·Radiation Oncology·Chun-Te WuChing-Chuan Hsieh
Sep 17, 2013·PloS One·Yusuke S HoriYoshiyuki Horio
Jun 17, 2014·PloS One·Stephanie E BrockRobert A Mitchell
Feb 14, 2013·Viruses·Federico De Marco
Jul 25, 2012·Radiation and Environmental Biophysics·Christopher C PerryJamie R Milligan
May 9, 2006·FEBS Letters·Aurora M Nedelcu
May 25, 2011·Mutation Research·Ingrit HamannAndrea Hartwig
Aug 25, 2010·International Journal of Radiation Oncology, Biology, Physics·Miao-Fen ChenJeng-Yi Wang
Mar 3, 2010·Toxicology and Applied Pharmacology·Daigo SumiYoshito Kumagai
Apr 3, 2007·Biochemical and Biophysical Research Communications·Sunil UpadhyayDavid Sidransky
Nov 7, 2006·International Journal of Radiation Oncology, Biology, Physics·Miao-Fen ChenKuan-Der Lee
Sep 1, 2005·Alcoholism, Clinical and Experimental Research·Heidi JänkäläTiina Mäki
Nov 14, 2012·Basic & Clinical Pharmacology & Toxicology·Vaiyapuri S Periasamy, Ali A Alshatwi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved