Role of P63 (CKAP4) in binding of surfactant protein-A to type II pneumocytes.

American Journal of Physiology. Lung Cellular and Molecular Physiology
Sandra R BatesAron B Fisher

Abstract

We have recently described a putative receptor for lung surfactant protein-A (SP-A) on rat type II pneumocytes. The receptor, P63, is a 63-kDa type II transmembrane protein. Coincubation of type II cells with P63 antibody (Ab) reversed the inhibitory effect of SP-A on secretagogue-stimulated surfactant secretion from type II cells. To further characterize SP-A interactions with P63, we expressed recombinant P63 protein in Escherichia coli and generated antibodies to P63. Immunogold electron microscopy confirmed endoplasmic reticulum and plasma membrane localization of P63 in type II cells with prominent labeling of microvilli. Binding characteristics of iodinated SP-A to type II cells in the presence of P63 Ab were determined. Binding (4 degrees C, 1 h) of (125)I-SP-A to type II cells demonstrated both specific (calcium-dependent) and nonspecific (calcium-independent) components. Ab to P63 protein blocked the specific binding of (125)I-SP-A to type II cells and did not change the nonspecific SP-A association. A549 cells, a pneumocyte model cell line, expressed substantial levels of P63 and demonstrated specific binding of (125)I-SP-A that was inhibited by the P63 Ab. The secretagogue (cAMP)-stimulated increase in calcium-depend...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J R WrightS Hawgood
Mar 1, 1989·Journal of Applied Physiology·S L YoungJ A Clements
Oct 1, 1989·Experimental Cell Research·J M CheekE D Crandall
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y KurokiD R Voelker
Apr 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R M RyanJ A Whitsett
Apr 4, 1986·Science·M S Brown, J L Goldstein
Apr 14, 1987·Biochemical and Biophysical Research Communications·K SanoD Voelker
Dec 1, 1981·Agents and Actions·G MilonP Truffa-Bachi
Mar 1, 1993·The American Journal of Physiology·M R ChinoyA B Fisher
Jul 10, 1996·Experimental Cell Research·D S StrayerA Chander
Jul 5, 1996·The Journal of Biological Chemistry·Z C ChroneosV L Shepherd
Oct 11, 1996·The Journal of Biological Chemistry·Q ChenA B Fisher
Mar 1, 1997·The American Journal of Physiology·M IkegamiA H Jobe
Aug 5, 1998·The American Journal of Physiology·Q ChenS R Bates
Aug 12, 1998·The American Journal of Physiology·M IkegamiA H Jobe
Nov 14, 1998·Biochimica Et Biophysica Acta·F X McCormack
Jun 1, 2000·Biochemistry·M L RuanoC Casals
Jan 3, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·P A StevensK P Zimmer
Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K R Khubchandani, J M Snyder
Mar 4, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Peter RückertAron B Fisher
Apr 5, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Deepika JainAron B Fisher
Jul 29, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sandra R BatesAron B Fisher
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Dec 10, 2003·The Lancet Oncology·J C CoffeyH P Redmond
Jul 12, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Deepika JainSandra R Bates
Oct 11, 2005·Molecular Immunology·Uday KishoreTrinad Chakraborty
Mar 25, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nisha GuptaSandra R Bates
Dec 18, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sandra R BatesAron B Fisher

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Citations

Sep 12, 2012·The Journal of Infectious Diseases·Mariette BarbierSebastián Albertí
Apr 13, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Aron B FisherSandra R Bates
Sep 28, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Altaf S KaziSandra R Bates
Jan 8, 2010·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zissis C ChroneosVirginia L Shepherd
Jan 8, 2010·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Sandra R Bates
Dec 3, 2003·American Journal of Respiratory Cell and Molecular Biology·Davey L PoelmaJ Freek van Iwaarden
May 20, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shuang-Xi LiHong-Yang Wang
Jan 26, 2010·Toxicology Letters·Yolanda I ChirinoClaudia M García-Cuellar
Jan 25, 2012·Journal of Applied Toxicology : JAT·Delia CavalloSergio Iavicoli
Mar 11, 2016·Genes & Genetic Systems·Félix F González-NavarroGabriel A López-Morteo
Jun 21, 2016·The Journal of Clinical Investigation·Hirokazu KimuraAkira Kikuchi
Feb 23, 2021·Frontiers in Molecular Biosciences·Shuang-Xi LiZhi-Yong Guo

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