Role of PCK1 gene on oil tea-induced glucose homeostasis and type 2 diabetes: an animal experiment and a case-control study

Nutrition & Metabolism
Qiantu HuRui Lin

Abstract

Oil tea is a type of traditional tea beverage used for treating various ailments in minority population in Guangxi, China. Our previous study showed oil tea improved glucose and lipid levels in type 2 diabetic mice. Yet, the underling molecular mechanisms are still not understood. This study aimed at assessing the effect of oil tea on glucose homeostasis and elucidating the molecular mechanisms underlying the oil tea-induced antidiabetic effects. Twenty seven db/db mice were gavaged with saline, metformin and oil tea for 8 weeks with measurement of biochemical profiles. A real-time2 (RT2) profiler polymerase chain reaction (PCR) array comprising 84 genes involved in glucose metabolism was measured and validated by quantitative PCR (qPCR). The association between the candidate genes and type 2 diabetes were further analyzed in a case-control study in the Chinese minority population. Oil tea treatment facilitated glucose homeostasis by decreasing fasting blood glucose and total cholesterol, and improving glucose tolerance. Suppressing phosphoenolpyruvate carboxykinase 1 (PCK1) expression was observed in the oil tea treatment group and the expression was significantly correlated with fasting blood glucose levels. Target prediction...Continue Reading

References

Sep 1, 1988·Diabetes·R S SurwitM N Feinglos
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·A ValeraF Bosch
Jan 1, 1997·Annual Review of Biochemistry·R W Hanson, L Reshef
Feb 12, 1998·The Journal of Clinical Investigation·D E ClouthierR E Hammer
Sep 15, 2001·Nature·A Vidal-Puig, S O'Rahilly
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 27, 2005·Current Drug Targets. Immune, Endocrine and Metabolic Disorders·P G Quinn, D Yeagley
Aug 22, 2007·Cell Biochemistry and Biophysics·Elmus G BealeClaude Forest
Mar 25, 2009·Journal of General Internal Medicine·Yali JingDalong Zhu
Jan 31, 2013·Archives of Pharmacal Research·Kyoung-Jin OhSeung-Hoi Koo
Mar 22, 2013·Journal of Medicinal Food·Silvia WeinSiegfried Wolffram
May 16, 2013·Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·Shi-Lin ChenZhong-Zhi Qian
Jun 28, 2013·The American Journal of Clinical Nutrition·Kai LiuMan-Tian Mi
Jun 29, 2013·Diabetes & Metabolism Journal·Ki-Cheor BaeDae-Kyu Song
Dec 18, 2013·The British Journal of Nutrition·Wan-Shui YangXin Wang
Jun 18, 2015·Critical Reviews in Eukaryotic Gene Expression·Muhammad Sajid Hamid AkashShuqing Chen

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Methods Mentioned

BETA
ELISA
PCR
genotyping
Assay

Software Mentioned

PLINK
GEne SeT AnaLysis Toolkit
WebGestalt
STATA
MassARRAY Assay Design

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