Role of pectin methylesterases in cellular calcium distribution and blossom-end rot development in tomato fruit

The Plant Journal : for Cell and Molecular Biology
Sergio Tonetto de FreitasElizabeth J Mitcham

Abstract

Blossom-end rot (BER) in tomato fruit (Solanum lycopersicum) is believed to be a calcium (Ca²⁺) deficiency disorder, but the mechanisms involved in its development are poorly understood. Our hypothesis is that high expression of pectin methylesterases (PMEs) increases Ca²⁺ bound to the cell wall, subsequently decreasing Ca²⁺ available for other cellular functions and thereby increasing fruit susceptibility to BER. The objectives of this study were to evaluate the effect of PME expression, and amount of esterified pectins and Ca²⁺ bound to the cell wall on BER development in tomato fruit. Wild-type and PME-silenced tomato plants were grown in a greenhouse. At full bloom, flowers were pollinated and Ca²⁺ was no longer provided to the plants to induce BER. Our results show that suppressing expression of PMEs in tomato fruit reduced the amount of Ca²⁺ bound to the cell wall, and also reduced fruit susceptibility to BER. Both the wild-type and PME-silenced fruit had similar total tissue, cytosolic and vacuolar Ca²⁺ concentrations, but wild-type fruit had lower water-soluble apoplastic Ca²⁺ content and higher membrane leakage, one of the first symptoms of BER. Our results suggest that apoplastic water-soluble Ca²⁺ concentration influ...Continue Reading

References

Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
Feb 1, 1997·Critical Reviews in Food Science and Nutrition·B R ThakurA K Handa
Sep 27, 2002·Journal of Experimental Botany·Lucille Alexander, Don Grierson
Aug 23, 2003·Annals of Botany·Philip J White, Martin R Broadley
Sep 18, 2004·Plant Physiology·Kendal D Hirschi
Jan 12, 2005·Annals of Botany·Lim C Ho, Philip J White
Aug 3, 2005·The Plant Cell·Peter K Hepler
Dec 3, 2005·The Plant Cell·Maurice Bosch, Peter K Hepler
Dec 1, 1990·Plant Physiology·A D CampbellJ M Labavitch
Aug 25, 2009·Journal of Experimental Botany·Federico MartinelliAbhaya M Dandekar
Oct 15, 2009·Molecular Plant·Sebastian WolfJérome Pelloux
Apr 10, 2010·Journal of Integrative Plant Biology·Peter K Hepler, Lawrence J Winship

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Citations

Jan 12, 2013·Journal of Experimental Botany·Sarika GuptaAaron M Rashotte
Jul 25, 2014·Journal of Experimental Botany·Fabien SénéchalJérôme Pelloux
Oct 13, 2019·Physiologia Plantarum·Frank FörsteKirsten Krause
Jun 17, 2020·Critical Reviews in Food Science and Nutrition·Xin FengPeiqiang Yu
Nov 22, 2018·Frontiers in Plant Science·Hui-Chen WuTsung-Luo Jinn
Jun 16, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yasin TopcuEsther van der Knaap
Oct 12, 2021·Frontiers in Plant Science·Lina QiuHaiyong Qu

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