Role of Periostin in Adhesion and Migration of Bone Remodeling Cells

PloS One
T CoboA Obaya

Abstract

Periostin is an extracellular matrix protein highly expressed in collagen-rich tissues subjected to continuous mechanical stress. Functionally, periostin is involved in tissue remodeling and its altered function is associated to numerous pathological processes. In orthodontics, periostin plays key roles in the maintenance of dental tissues and it is mainly expressed in those areas where tension or pressing forces are taking place. In this regard, high expression of periostin is essential to promote migration and proliferation of periodontal ligament fibroblasts. However little is known about the participation of periostin in migration and adhesion processes of bone remodeling cells. In this work we employ the mouse pre-osteoblastic MC3T3-E1 and the macrophage-like RAW 264.7 cell lines to overexpress periostin and perform different cell-based assays to study changes in cell behavior. Our data indicate that periostin overexpression not only increases adhesion capacity of MC3T3-E1 cells to different matrix proteins but also hampers their migratory capacity. Changes on RNA expression profile of MC3T3-E1 cells upon periostin overexpression have been also analyzed, highlighting the alteration of genes implicated in processes such as ...Continue Reading

References

Jun 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L D QuarlesR J Wenstrup
Jul 15, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K HoriuchiA Kudo
Mar 5, 2002·The Journal of Endocrinology·M R SchneiderH Lahm
Feb 27, 2003·Breast Cancer Research and Treatment·Hidefumi SasakiAnthony Elias
May 8, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jelica Gluhak-HeinrichDubravko Pavlin
Apr 22, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tetsuro NakazawaMasashi Yamazaki
Sep 17, 2004·Journal of Cell Science·Cai HuangMichael D Schaller
Sep 24, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Hironobu SuzukiTakeyasu Maeda
Oct 30, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Volkhard LindnerCalvin P H Vary
Nov 13, 2004·Biology of Reproduction·Isabelle Dorval-CoiffecClaire Piquet-Pellorce
Mar 25, 2005·Journal of Cell Science·Elizabeth G Canty, Karl E Kadler
Nov 5, 2005·The Journal of Biological Chemistry·Jiliang LiCharles H Turner
Jul 4, 2006·The Journal of Allergy and Clinical Immunology·Go TakayamaKenji Izuhara
Jan 18, 2007·Journal of Cellular Biochemistry·Russell A NorrisRoger R Markwald
Sep 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Prescott G WoodruffJohn V Fahy
Nov 14, 2008·Journal of Cellular Physiology·Shimei ZhuJudith Litvin
Mar 25, 2009·Cellular and Molecular Life Sciences : CMLS·Kai RuanGaoliang Ouyang
Jun 23, 2009·Journal of Cell Communication and Signaling·Linda Jackson-BoetersDouglas W Hamilton
Feb 26, 2010·The Journal of Biological Chemistry·Takumi MaruhashiAkira Kudo
Dec 7, 2010·The Journal of Biological Chemistry·Dedong MaJian Q Feng
Aug 13, 2011·Cellular and Molecular Life Sciences : CMLS·Akira Kudo

❮ Previous
Next ❯

Citations

Feb 26, 2013·PloS One·Sara RezaMaria Mahmud
Jan 18, 2017·Expert Review of Hematology·Maria GavriatopoulouEvangelos Terpos
Mar 30, 2018·International Journal of Molecular Sciences·Francesca DiomedeEmanuela Mazzon
Sep 11, 2017·Cellular and Molecular Life Sciences : CMLS·Juan Du, Minqi Li
Oct 18, 2018·American Journal of Physiology. Renal Physiology·Archana RamanDarren P Wallace
Apr 18, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lei CaiMuhammad Farooq Rai
Sep 12, 2018·Clinical Sarcoma Research·Jennifer M BrownNick Athanasou

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE66416

Methods Mentioned

BETA
inmunoprecipitation
electrophoresis
fluorescence microscopy
PCR
transfect
transfection
RNA array

Software Mentioned

GraphPad Prism
Image J
Affymetrix Expression Console

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.