Role of PERK-eIF2α signaling pathway in fetal male rats with hypospadias induced by di-n-butyl phthalate

The Kaohsiung Journal of Medical Sciences
Zhang ZhaoWen Fu

Abstract

This study aims to explore the role of PERK-eIF2α signaling pathway in fetal male rats with hypospadias induced by maternal exposure to di-n-butyl phthalate (DBP). DBP was used to treat pregnant SD rats by gastric intubation from gestation day (GD) 14-18 to construct a hypospadias rat model. The amount, weight, anogenital distance (AGD), and hypospadias incidence of rats were recorded and the genital tubercle (GT) of fetal male rats was collected on GD 19. Western blotting was performed to detect the expressions of PERK-eIF2α pathway- and autophagy-related proteins, and cell apoptosis was detected using TUNEL method. Then, GT fibroblasts of fetal rats were obtained and transfected with PERK-siRNA to detect cell apoptosis and autophagy in each transfected group. The incidence of hypospadias was 43.49% in fetal male rats induced by DBP. The fetal rats in DBP group presented the decreased birth weight and anogenital distance (AGD)/body weight ratio than the Control group (all P < 0.05). Further, p-PERK, p-eIF2α and ATF4 protein expressions and the ratio of LC3-II/LC3-I were greatly increased in the GTs of fetal rats, while apoptosis index (AI) and P62 protein expression were evidently decreased (all P < 0.05). In addition, the apo...Continue Reading

References

Feb 8, 2000·Teratology·J F van der WerffC Vermeij-Keers
Jul 14, 2000·Molecular Endocrinology·E B RuckerL Hennighausen
Sep 7, 2002·Human Genetics·Louise FredellAgneta Nordenskjöld
Jun 5, 2003·Development·Emily A MorganH Scott Stadler
May 14, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kim P LehmannKevin W Gaido
Mar 5, 2008·International Journal of Andrology·S ChristiansenU Hass
Apr 22, 2008·Methods in Molecular Biology·Isei TanidaEiki Kominami
Jul 29, 2008·Human Genetics·Hanh T T ThaiAgneta Nordenskjöld
Jun 16, 2009·Cell·Jorge Moscat, Maria T Diaz-Meco
Jul 1, 2010·Birth Defects Research. Part A, Clinical and Molecular Teratology·Lei JinAiguo Ren
Sep 3, 2010·Nature Cell Biology·Noboru Mizushima, Beth Levine
Nov 30, 2010·International Journal of Endocrinology·Chunjiong WangJichun Yang
May 11, 2012·Journal of Pediatric Endocrinology & Metabolism : JPEM·Alexis Parada-BustamanteAndrea Castro
Dec 6, 2012·The Journal of Biological Chemistry·Lei QiangYu-Ying He
Aug 27, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi-Jen ChenYu-Jen Wu
Sep 7, 2013·Journal of the American Heart Association·Masashi MasudaMakoto Miyazaki
Sep 10, 2014·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Yang HuDongmei Li

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