Role of peroxynitrite and recombinant human manganese superoxide dismutase in reducing ischemia-reperfusion renal tissue injury

Transplantation Proceedings
N A RahmanC Ohyama

Abstract

In an acute kidney transplant rejection rat model, we demonstrated that manganese superoxide dismutase (MnSOD) activity was significantly reduced and MnSOD was nitrated by peroxynitrite (ONOO(-)), resulting in tissue injury. We examined whether tissue injury was reduced after external supplementation of recombinant human MnSOD in a rat renal ischemia-reperfusion injury model. Male Brown-Norway rats underwent dissection of the right kidney. The animals were divided into 3 groups. The controls had the left renal blood vessels clamped for 90 minutes to induce ischemia, followed by reperfusion for 16 hours. In the intraperitoneal administration group, MnSOD was administered 30 minutes before ischemia and immediately before reperfusion. In the sham group, neither ischemia nor reperfusion was performed. After reperfusion, blood was collected, the left kidney was dissected and renal function and tissue injury were evaluated. Serum creatinine and K(+), blood urea nitrogen, and aspartate aminotransferase activity decreased significantly, whereas serum Na(+) and renal function improved in the MnSOD group compared with the control and sham groups. On hematoxylin and eosin staining, the histological score indicated that acute tubular necro...Continue Reading

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Citations

Jun 29, 2013·Clinical and Experimental Nephrology·Antonio PisaniAldo Mancini
Sep 20, 2012·Journal of Hepatology·Maeva GuillaumeJoan Carles Garcia-Pagán
Sep 4, 2015·PloS One·Bo ZhangWen-Quan Zou
Dec 1, 2012·American Journal of Physiology. Renal Physiology·Nirmala Parajuli, Lee Ann MacMillan-Crow
Nov 17, 2019·International Journal of Molecular Sciences·Siniša ĐuraševićZoran Todorović
Mar 23, 2017·Transplantation·Dane HoeksmaHenri G D Leuvenink

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