PMID: 9421858Jan 9, 1998Paper

Role of peroxynitrite in the protein oxidation and apoptotic DNA fragmentation in vascular smooth muscle cells stimulated with bacterial lipopolysaccharide and interferon-gamma

Shock
M O'ConnorC Szabó

Abstract

In the present study, we investigated the role of endogenous and exogenous peroxynitrite in the process of DNA fragmentation and protein oxidation in cultured rat aortic smooth muscle cells. Peroxynitrite induced DNA fragmentation over a 24 hr period. The effect of peroxynitrite was unaffected by pretreatment with 3-aminobenzamide, an inhibitor of the nuclear enzyme poly (ADP-ribose) synthetase (PARS). Stimulation of the smooth muscle cells with bacterial lipopolysaccharide and interferon-gamma produced nitric oxide and peroxynitrite, and resulted in a significant degree of apoptotic DNA fragmentation. The nitric oxide synthase inhibitor NG-methyl-L-arginine (3 mM), but not the PARS inhibitor 3-aminobenzamide (1 mM), reduced the DNA fragmentation. Stimulation with bacterial lipopolysaccharide and interferon-gamma also caused a marked oxidation of proteins in the smooth muscle cells, which was inhibited by NG-methyl-L-arginine, as well as by the superoxide dismutase mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin. Based on these data, we propose a role for peroxynitrite-mediated, PARS-independent pathways in the apoptotic process and in the protein oxidation in bacterial lipopolysaccharide and interferon-gamma-stimulated smoo...Continue Reading

Citations

Jul 31, 1998·European Journal of Pharmacology·C Szabó
May 23, 2002·Biochimie·Christophe FleuryJean-Luc Vayssière
Mar 17, 2007·Circulation Research·George Tellides, Jordan S Pober
Jul 4, 2006·The Journal of Pharmacology and Experimental Therapeutics·Salvatore CuzzocreaDaniela Salvemini

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