Role of pH on the calcium ion dependence of the nitric oxide synthase in the carp brain

Brain Research Bulletin
Angela Conte

Abstract

The role of pH on the calcium dependence of nitric oxide synthase (NOS) of Cyprinus carpio brain was investigated. This fish is known to survive prolonged periods of hypoxia. Under this condition, cerebral blood flow is no longer regulated by nitric oxide (NO). Nitric oxide synthase activity is pH dependent in the range of pH between 7.4 and 6.2 with a decrease when tissue acidifies. At acidic pH, the dependence of the NOS activity on the free Ca(2+) concentrations changes considerably and shows an EC(50) of 0.13 microM at pH 7.1 and of 5.1 microM at pH 6.2 for the soluble enzyme. The variation in the Ca(2+) dependence with acidification is greater for the soluble than for the particulate enzyme. This may be the main factor protecting sudden NO formation mainly during anoxic-normoxic transitions.

References

May 3, 1990·Nature·J S Beckman
Dec 1, 1989·Naunyn-Schmiedeberg's Archives of Pharmacology·A MülschR Busse
Jan 1, 1988·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·G E Nilsson
Jan 1, 1984·Journal of Receptor Research·P J Marangos
Mar 1, 1994·The Biochemical Journal·R G Knowles, S Moncada
Feb 28, 1994·Neuroscience Letters·T OstholmP Ekström
Apr 25, 1994·Neuroscience Letters·B I HolmqvistP Ekström
Jan 1, 1994·Annual Review of Biochemistry·D S Bredt, S H Snyder
Oct 8, 1994·The Journal of Comparative Neurology·G BrüningB Mayer
May 1, 1995·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·P Hylland, G E Nilsson
May 29, 1995·FEBS Letters·A Conte, E Ottaviani
Aug 8, 1993·The Journal of Comparative Neurology·G Brüning
Jan 1, 1996·Current Topics in Cellular Regulation·D A WinkJ B Mitchell
Jun 21, 1996·The Journal of Biological Chemistry·U A ArnetC J Lowenstein
Jan 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S C Wood, R Gonzales
Mar 1, 1997·Trends in Neurosciences·C Iadecola
Apr 8, 1998·Neuroscience Letters·A Conte, E Ottaviani
Apr 30, 1998·The Biochemical Journal·A C GorrenB Mayer
Jun 5, 1999·Brain Research Bulletin·A R JayakumarR Jayakumar
Feb 22, 2000·Behavioural Brain Research·A D Székely
May 16, 2000·Brain Research Bulletin·E Picano, M P Abbracchio
Mar 29, 2001·Brain Research Bulletin·R E HarlanM M Garcia

❮ Previous
Next ❯

Citations

Mar 12, 2008·Biochimica Et Biophysica Acta·Liubov Lemberskiy-KuzinZvi Priel
Jun 5, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sandrine Arbogast, Michael B Reid

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.