Role of phosphodiesterase in the parathormone-stimulated adenosine 3',5'-monophosphate response in bone cell populations enriched in osteoclasts and osteoblasts

Endocrinology
R L Jilka, D V Cohn

Abstract

The stimulation of cAMP formation by parathormone was assessed in isolated bone cell populations enriched in either osteoclast-like (CL) or osteoblast-like (BL) cells. In the presence of the potent phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (IBMX), at 37 C, the CL cells produced at least 20% the amount of cAMP formed by the BL cells, but only about 5% when the inhibitor was omitted. The rate of cAMP production in response to parathormone was measured at 23 C in order to obtain a better indication of the capacity of the cells to generate this nucleotide. Under these conditions, the CL cells produced cAMP at a rate of 50-60% that of the BL cells regardless of whether IBMX was present. The phosphodiesterase activity in CL cell extracts was twice that in BL cell extracts, and IBMX inhibited this activity. These results show that when an effective inhibitor of phosphodiesterase is present, the CL cells exhibit a significant response to parathormone in terms of cAMP production. The possibility that the cAMP response in the CL cells was due to contaminating BL cells (which would have to be about 50% of the total cell number) was ruled out by the demonstration that citrate decarboxylating activity, a marker for the BL cel...Continue Reading

Citations

Mar 27, 2012·The Journal of Biological Chemistry·Melissa A KacenaAngela Bruzzaniti
Jan 1, 1989·Connective Tissue Research·M C HorowitzR L Jilka
Sep 17, 2015·Journal of Cellular Biochemistry·Tomas E MeijomeMelissa A Kacena
Sep 11, 2014·Journal of Cellular Physiology·Tomas E MeijomeMelissa A Kacena
Aug 28, 2014·Journal of Cellular Physiology·Ying-Hua ChengMelissa A Kacena
May 5, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ying-Hua ChengMelissa A Kacena
May 28, 2010·Journal of Cellular Biochemistry·Brahmananda R ChittetiMelissa A Kacena
Jan 31, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ying-Hua ChengMelissa A Kacena
Jan 7, 2010·Journal of Cellular Biochemistry·Wendy A CiovaccoMelissa A Kacena
Aug 1, 1986·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·J StanfeldZ Davidovitch
May 1, 1989·Toxicology and Applied Pharmacology·J F Rosen, J G Pounds
Nov 11, 2015·Journal of Cellular Biochemistry·Pierre P ElenisteAngela Bruzzaniti
Jan 10, 2017·Journal of Cellular Biochemistry·David J OlivosMelissa A Kacena
Jul 17, 2018·Journal of Cellular Biochemistry·David J OlivosLindsey D Mayo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.