PMID: 11607537May 9, 1995Paper

Role of phosphorylation in elicitation of the oxidative burst in cultured soybean cells

Proceedings of the National Academy of Sciences of the United States of America
S Chandra, P. S. Low

Abstract

The oxidative burst is likely the most rapid defense response mounted by a plant under pathogen attack, and the generated oxidant species may be essential to several subsequent defense responses. In our effort to characterize the signal-transduction pathways leading to rapid H2O2/O2- biosynthesis, we have examined the role of protein phosphorylation in this resistance mechanism. K-252a and staurosporine, two protein-kinase inhibitors, were found to block the oxidative burst in a concentration-dependent manner. When added during H2O2 generation, the burst was observed to rapidly terminate, suggesting that continuous phosphorylation was essential for its maintenance. Importantly, phosphatase inhibitors (calyculin A and okadaic acid) were found to induce the oxidative burst in the absence of any additional stimulus. This may suggest that certain kinases required for the burst are constitutively active and that stabilization of the phosphorylated forms of their substrates is all that is required for burst activity. In autoradiographs of elicited and unstimulated cells equilibrated with 32PO4(3-), several phosphorylated polypeptide bands were revealed that could represent proteins essential for the burst.

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R J Anderberg, M K Walker-Simmons
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J C SteinJ B Nasrallah
Mar 31, 1989·Biochemical and Biophysical Research Communications·H IshiharaD Uemura
Sep 1, 1986·Archives of Biochemistry and Biophysics·P S Low, P F Heinstein
Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P ReymondK L Poff
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G FelixT Boller

❮ Previous
Next ❯

Citations

Apr 2, 2005·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·Isak B Gerber, Ian A Dubery
Nov 11, 1998·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·D PontierD Roby
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Mar 14, 2007·Annual Review of Phytopathology·Ralph Hückelhoven
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·S ChandraP S Low
Mar 13, 2012·Annual Review of Plant Biology·Benjamin Schwessinger, Pamela C Ronald
Aug 12, 2000·Molecular Plant-microbe Interactions : MPMI·F Lecourieux-OuakedA Lebrun-Garcia
Jun 2, 2001·Molecular Plant-microbe Interactions : MPMI·H YoshiokaN Doke
Jul 27, 2007·The Plant Journal : for Cell and Molecular Biology·Thomas S NühseScott C Peck
Aug 2, 2016·Nature Reviews. Immunology·Daniel Couto, Cyril Zipfel
Jul 4, 2006·Plant & Cell Physiology·Kaori KojoKoh Iba
May 18, 2013·International Journal of Molecular Sciences·Joel Fürstenberg-HäggSøren Bak
Aug 3, 2014·The EMBO Journal·Cécile SegonzacCyril Zipfel
Jun 27, 2019·International Journal of Molecular Sciences·Csaba MáthéMárta M-Hamvas
Nov 10, 1998·The Plant Journal : for Cell and Molecular Biology·A Lebrun-GarciaA Pugin
Jul 1, 2008·Functional Plant Biology : FPB·Damien J LightfootAmanda J Able
Aug 3, 2002·Plant & Cell Physiology·Kenji UmemuraHideki Usami
Jan 26, 2008·The Journal of Biological Chemistry·Yoko OgasawaraKazuyuki Kuchitsu
Jul 15, 1998·Plant Physiology·M J StennisP S Low
Mar 10, 1998·Plant Physiology·A C CazaléC Laurière
Aug 8, 1997·Biochemical and Biophysical Research Communications·A T Schroeder Taylor, P S Low
Jan 7, 2004·Journal of Agricultural and Food Chemistry·Franco Gozzo

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.