Role of Piezo Channels in Ultrasound-stimulated Dental Stem Cells

Journal of Endodontics
Qianhua GaoBen A Scheven

Abstract

Piezo1 and Piezo2 are mechanosensitive membrane ion channels. We hypothesized that Piezo proteins may play a role in transducing ultrasound-associated mechanical signals and activate downstream mitogen-activated protein kinase (MAPK) signaling processes in dental cells. In this study, the expression and role of Piezo channels were investigated in dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) after treatment with low-intensity pulsed ultrasound (LIPUS). Cell proliferation was evaluated by bromodeoxyuridine incorporation. Western blots were used to analyze the proliferating cell nuclear antigen as well as the transcription factors c-fos and c-jun. Enzyme-linked immunosorbent assay and Western blotting were used to determine the activation of MAPK after LIPUS treatment. Ruthenium red (RR), a Piezo ion channel blocker, was applied to determine the functional role of Piezo proteins in LIPUS-stimulated cell proliferation and MAPK signaling. Western blotting showed the presence of Piezo1 and Piezo2 in both dental cell types. LIPUS treatment significantly increased the level of the Piezo proteins in DPSCs after 24 hours; however, no significant effects were observed in PDLSCs. Treatment with RR significant...Continue Reading

Citations

Oct 12, 2018·Stem Cells International·Huaming HuangYan-Heng Zhou
Aug 28, 2019·Annual Review of Pharmacology and Toxicology·Bailong Xiao
Aug 9, 2020·Frontiers in Physiology·Songyun WangHong Jiang
Mar 28, 2020·Cell and Tissue Research·Abolfazl BarzegariGraciela Pavon-Djavid
Sep 23, 2020·Genes·Beatriz de LucasBeatriz G Gálvez
Dec 12, 2018·Frontiers in Physiology·Massimo MarrelliFrancesco Paduano
Dec 10, 2020·Cellular Signalling·Fang PeiZhihe Zhao
Mar 3, 2021·Archives of Oral Biology·Jeeranan ManokawinchokeHiroshi Egusa
Apr 14, 2021·American Journal of Hematology·Nicolas JankovskyLoïc Garçon
Nov 13, 2021·Chemical Reviews·Athanasios G AthanassiadisPeer Fischer

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