Role of PKCα-p(38)MAPK-G(i)α axis in NADPH oxidase derived O(2)(·-)-mediated activation of cPLA(2) under U46619 stimulation in pulmonary artery smooth muscle cells

Archives of Biochemistry and Biophysics
Sajal ChakrabortiTapati Chakraborti

Abstract

We have recently reported that treatment of bovine pulmonary artery smooth muscle cells with the thromboxane A(2) mimetic, U46619 stimulated NADPH oxidase derived O(2)(·-) level, which subsequently caused marked increase in [Ca(2+)](i)[17]. Herein, we demonstrated that O(2)(·-)-mediated increase in [Ca(2+)](i) stimulates an aprotinin sensitive proteinase activity, which proteolytically activates PKC-α under U46619 treatment to the cells. The activated PKC-α then phosphorylates p(38)MAPK and that subsequently caused G(i)α phosphorylation leading to stimulation of cPLA(2) activity in the cell membrane.

References

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F HirataJ Axelrod
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Feb 1, 1989·Physical Review A: General Physics·G YuR J Maurer
Feb 1, 2003·Biochemical and Biophysical Research Communications·S J PersaudP E Squires
Jun 30, 2007·The Journal of Pharmacology and Experimental Therapeutics·Sandra ClaroRaouf Ali Khalil

❮ Previous
Next ❯

Citations

Nov 15, 2013·Scandinavian Journal of Medicine & Science in Sports·D van de PolM Maas
Nov 17, 2014·Molecular and Cellular Biochemistry·Sajal ChakrabortiTapati Chakraborti
Aug 7, 2017·Molecular and Cellular Biochemistry·Sajal ChakrabortiTapati Chakraborti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomegaly

Cardiomegaly, known as an enlarged heart, is a multifactorial disease with different pathophysiological mechanisms. Hypertension, pregnancy, exercise-induced and idiopathic causes are some mechanisms of cardiomegaly. Discover the latest research of cardiomegaly here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.