Role of Plasmodium berghei cGMP-dependent protein kinase in late liver stage development.

The Journal of Biological Chemistry
Adebola FalaePurnima Bhanot

Abstract

The liver is the first organ infected by Plasmodium sporozoites during malaria infection. In the infected hepatocytes, sporozoites undergo a complex developmental program to eventually generate hepatic merozoites that are released into the bloodstream in membrane-bound vesicles termed merosomes. Parasites blocked at an early developmental stage inside hepatocytes elicit a protective host immune response, making them attractive targets in the effort to develop a pre-erythrocytic stage vaccine. Here, we generated parasites blocked at a late developmental stage inside hepatocytes by conditionally disrupting the Plasmodium berghei cGMP-dependent protein kinase in sporozoites. Mutant sporozoites are able to invade hepatocytes and undergo intracellular development. However, they remain blocked as late liver stages that do not release merosomes into the medium. These late arrested liver stages induce protection in immunized animals. This suggests that, similar to the well studied early liver stages, late stage liver stages too can confer protection from sporozoite challenge.

References

Feb 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elodie BelnoueLaurent Rénia
Apr 24, 2004·Trends in Parasitology·David A Baker, John M Kelly
Dec 14, 2004·The Journal of Biological Chemistry·Purnima BhanotVictor Nussenzweig
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ann-Kristin MuellerStefan H I Kappe
Aug 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Melissa R van DijkChris J Janse
Dec 6, 2005·Molecular and Biochemical Parasitology·Carmen A DiazPaul A Liberator
Oct 17, 2006·Nature Reviews. Microbiology·Miguel PrudêncioMaria M Mota
Jan 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Alice S TarunStefan H I Kappe
Sep 26, 2008·Parasite : Journal De La Société Française De Parasitologie·L Rénia
Dec 11, 2008·Cellular Microbiology·Ashley M VaughanStefan H I Kappe
Feb 20, 2009·Malaria Journal·Mary R Galinski, John W Barnwell
Apr 22, 2009·Cell Host & Microbe·Audrey CombeRobert Ménard
May 15, 2009·Cellular Microbiology·Tomoko IshinoPatricia Baldacci
Jul 31, 2009·The New England Journal of Medicine·Meta RoestenbergRobert Sauerwein

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Citations

Apr 24, 2013·Trends in Parasitology·Krystelle Nganou-Makamdop, Robert W Sauerwein
Sep 3, 2011·Nature Protocols·Céline LacroixRobert Ménard
Jun 2, 2011·Molecular & Cellular Proteomics : MCP·Angela TrieuDenise L Doolan
Jul 21, 2010·Antimicrobial Agents and Chemotherapy·Dhruv Panchal, Purnima Bhanot
Dec 19, 2013·PLoS Pathogens·Catherine SuarezMichael J Blackman
Nov 16, 2011·Future Microbiology·Zhenyu LiuLiwang Cui
Sep 4, 2015·Cellular Microbiology·Sanna R RijpmaBlandine M Franke-Fayard
Mar 19, 2013·International Journal for Parasitology·Andreas NagelVolker Heussler
Sep 7, 2012·International Journal of Medical Microbiology : IJMM·Damien Jacot, Dominique Soldati-Favre
Mar 28, 2012·Molecular and Biochemical Parasitology·Dhruv PanchalPurnima Bhanot
Jul 9, 2011·Trends in Molecular Medicine·Steffen Borrmann, Kai Matuschewski
Dec 24, 2010·Cellular Microbiology·David A Baker
Aug 19, 2011·Molecular Microbiology·Joana M HaussigTaco W A Kooij
May 23, 2012·Microbes and Infection·Christine S HoppDavid A Baker
Jan 13, 2011·FEMS Microbiology Reviews·Matthew K Gould, Harry P de Koning
May 16, 2015·Nature Reviews. Microbiology·Tania F de Koning-WardBrendan S Crabb
Jul 29, 2010·Trends in Parasitology·Chris J JanseShahid M Khan
May 24, 2016·Molecular and Biochemical Parasitology·Teresa Gil CarvalhoChristian Doerig
Jun 15, 2011·Anais Da Academia Brasileira De Ciências·Silke LeutnerChristoph G Grevelding
Mar 1, 2017·Cold Spring Harbor Perspectives in Medicine·Ashley M Vaughan, Stefan H I Kappe
Dec 22, 2017·Open Biology·David A BakerEloise M Walker
Sep 17, 2013·Nature Reviews. Microbiology·Robert MénardRogerio Amino
Aug 29, 2013·Current Opinion in Infectious Diseases·Judith E Epstein, Thomas L Richie
May 31, 2019·Life Science Alliance·Hadi Hasan ChoudharySatish Mishra
Nov 28, 2012·Expert Review of Vaccines·Patrick E DuffyCharles Anderson
Oct 4, 2013·The Journal of Biological Chemistry·Lina TawkJean-Christophe Barale
Dec 1, 2017·PLoS Medicine·UNKNOWN malERA Refresh Consultative Panel on Basic Science and Enabling Technologies
Sep 2, 2018·Malaria Journal·Ross G DouglasFriedrich Frischknecht
Mar 18, 2020·Life Science Alliance·Konstantinos KoussisMichael J Blackman
Oct 21, 2017·Molecular Microbiology·Rama R YakubuNatalie C Silmon de Monerri
Jun 18, 2017·Frontiers in Cellular and Infection Microbiology·Oriana KreutzfeldKai Matuschewski

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