PMID: 9424405Mar 1, 1997Paper

Role of platelet activating factor, leukotrienes and polymorphs in the FMLP induced increase in microvascular leakage in rabbit trachea

Respirology : Official Journal of the Asian Pacific Society of Respirology
M J MathesonN Berend

Abstract

This study was designed to determine the time course of N-formyl-methionyl-leucyl-phenylalanine (FMLP) induced microvascular leakage in the airways of anaesthetized, tracheostomized New Zealand white rabbits. We have previously shown that FMLP increases microvascular leakage in the rabbit trachea at 30 min post challenge. A further aim was to determine the mechanisms underlying this response. Microvascular leakage was assessed using the albumin binding dye, Evans blue which was injected intravenously (50 mg/kg) immediately prior to FMLP challenge (10 mg nebulised for 2 min) or the control (dimethylsulphoxide/saline). Microvascular leakage was assessed in the trachea and bronchi at 15 min, 22.5 min, 45 min and 120 minutes with n = 6 for each group. The only significant difference between control and FMLP challenged groups was at 45 min in the bronchi (FMLP 77.6 +/- 12.2, control 33.4 +/- 5.7, P < 0.05). To determine the mechanism underlying FMLP-induced increases in microvascular leakage rabbits were treated with one of the following: (i) nitrogen mustard (1.75 mg/kg intravenously), which depletes circulating polymorphs (n = 6); (ii) the platelet activating factor (PAF) receptor antagonist WEB 2086 (10 mg/kg; n = 5); (iii) the C...Continue Reading

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Citations

Jun 9, 2004·Respirology : Official Journal of the Asian Pacific Society of Respirology·Melissa MathesonNorbert Berend
Jan 3, 2004·Nature Neuroscience·Sonia BishopAndrew D Lawrence

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