Role of Pneumococcal Autolysin for KLF4 Expression and Chemokine Secretion in Lung Epithelium

American Journal of Respiratory Cell and Molecular Biology
Janine ZahltenStefan Hippenstiel

Abstract

In severe pneumococcal pneumonia, the delicate balance between a robust inflammatory response necessary to kill bacteria and the loss of organ function determines the outcome of disease. In this study, we tested the hypothesis that Krueppel-like factor (KLF) 4 may counter-regulate Streptococcus pneumoniae-related human lung epithelial cell activation using the potent proinflammatory chemokine IL-8 as a model molecule. Pneumococci induced KLF4 expression in human lung, in primary human bronchial epithelial cells, and in the lung epithelial cell line BEAS-2B. Whereas proinflammatory cell activation depends mainly on the classical Toll-like receptor 2-mitogen-activated protein kinase or phosphatidylinositide 3-kinase and NF-κB pathways, the induction of KLF4 occurred independently of these molecules but relied, in general, on tyrosine kinase activation and, in part, on the src kinase family member yamaguchi sarcoma viral oncogene homolog (yes) 1. The up-regulation of KLF4 depended on the activity of the main pneumococcal autolysin LytA. KLF4 overexpression suppressed S. pneumoniae-induced NF-κB and IL-8 reporter gene activation and release, whereas small interfering RNA-mediated silencing of KLF4 or yes1 kinase led to an increase ...Continue Reading

References

Feb 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Richard MalleyDouglas T Golenbock
Mar 7, 2003·Genome Biology·Joanna KaczynskiRaul Urrutia
Jun 25, 2004·The Journal of Biological Chemistry·Bastian OpitzStefan Hippenstiel
Oct 16, 2004·The Journal of Biological Chemistry·Bernd SchmeckStefan Hippenstiel
Nov 13, 2004·FEMS Microbiology Reviews·Rubens López, Ernesto García
May 17, 2005·Nature Structural & Molecular Biology·Juan A HermosoMargarita Menéndez
Sep 20, 2005·The Journal of Biological Chemistry·Mark W FeinbergMukesh K Jain
Nov 22, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Bernd SchmeckStefan Hippenstiel
Sep 15, 2006·The European Respiratory Journal·B SchmeckS Hippenstiel
Sep 29, 2006·Cellular Microbiology·Barbara AlbigerBirgitta Henriques-Normark
Aug 28, 2007·Biochemical and Biophysical Research Communications·Junwen LiuXianzhong Xiao
Sep 1, 2007·The EMBO Journal·Mark W FeinbergMukesh K Jain
Oct 11, 2007·Respiratory Research·Pascal KnuefermannChristian Grohé
Apr 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonathan K AlderCurt I Civin
Nov 11, 2009·Molecular and Cellular Biology·Paul M EvansChunming Liu
Dec 9, 2009·Biochemical and Biophysical Research Communications·Guadalupe VillarrealGuillermo García-Cardeña
Jan 21, 2010·Expert Opinion on Pharmacotherapy·Stavros Anevlavis, Demosthenes Bouros
Jun 8, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Janine ZahltenStefan Hippenstiel
Jun 15, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Juan Carlos MonteroAtanasio Pandiella
Jun 15, 2011·The Journal of Clinical Investigation·Xudong LiaoMukesh K Jain
Jun 2, 2012·The European Respiratory Journal·Janine ZahltenStefan Hippenstiel
Nov 20, 2012·The Journal of Clinical Investigation·Guangjin ZhouMukesh K Jain
Dec 11, 2013·Biochemical and Biophysical Research Communications·Yan SunJin-kun Wen
May 27, 2014·Cell·Mohamed Lamkanfi, Vishva M Dixit

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Citations

Jun 8, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Lei Yu, ChunWen Li
Dec 7, 2016·Molecular Medicine Reports·Sara NovickYaffa Mizrachi Nebenzahl
Dec 22, 2016·Cell and Tissue Research·Andreas C HockeStefan Hippenstiel

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