Role of pneumococcal surface protein C in nasopharyngeal carriage and pneumonia and its ability to elicit protection against carriage of Streptococcus pneumoniae

Infection and Immunity
Priya BalachandranD E Briles

Abstract

Previous studies suggested that PspC is important in adherence and colonization within the nasopharynx. In this study, we conducted mutational studies to further identify the role PspC plays in the pathogenesis of pneumococci. pspC and/or pspA was insertionally inactivated in a serotype 2 Streptococcus pneumoniae strain and in a serotype 19 S. pneumoniae strain. In the mouse colonization model, pneumococcal strains with mutations in pspC were significantly attenuated in their abilities to colonize. In a mouse pneumonia model, strains with mutations in pspC were unable to infect or multiply within the lung. Using reverse transcriptase PCR we were able to demonstrate that pspC is actively transcribed in vivo, when the bacteria are growing in the nasal cavity and in the lungs. In the bacteremia model, a strain mutated for pspC alone behaved like the wild type, but the absence of both pspC and pspA caused accelerated clearance of the bacteria. Intranasal immunization with PspC with cholera toxin subunit B as an adjuvant protected against intranasal challenge. Evidence was also obtained that revertants that spontaneously acquired PspC expression could multiply and colonize the nasal tissue. This latter finding strongly indicates tha...Continue Reading

References

Jul 1, 1988·Reviews of Infectious Diseases·D E BrilesL S McDaniel
Dec 1, 1986·Journal of Bacteriology·J YotherD E Briles
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·L S HåvarsteinD A Morrison
Mar 1, 1995·Infection and Immunity·D R CundellElaine I Tuomanen
Feb 1, 1996·Molecular Microbiology·B SpellerbergH R Masure
Jan 1, 1997·Infection and Immunity·K TakashimaK Yamaguchi
Sep 18, 1997·The Journal of Infectious Diseases·I Idänpään-HeikkiläElaine I Tuomanen
Jan 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·D E BrilesMarilyn J Crain
Jul 3, 1998·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·J N Weiser
Aug 24, 1999·Infection and Immunity·A H TuAlexander J Szalai
Apr 14, 2000·The Journal of Infectious Diseases·M MadsenM K Hostetter
Jul 19, 2000·Infection and Immunity·K OverwegP W M Hermans
Sep 1, 2000·The Journal of Biological Chemistry·R JanulczykLars Björck
Sep 19, 2000·Infection and Immunity·K K GosinkH R Masure
Apr 9, 2001·Infection and Immunity·Anders P HakanssonD E Briles
Apr 9, 2001·Infection and Immunity·S DaveL S McDaniel
May 12, 2001·Infection and Immunity·A D Magee, J Yother

Citations

Jan 6, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Richard Malley
Mar 24, 2004·Mechanisms of Ageing and Development·D E Briles
Dec 13, 2012·The Journal of Biological Chemistry·Alan BassetRichard Malley
May 23, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Roger EaganS B Gordon
Oct 16, 2008·Clinical Microbiology Reviews·Damiana ChiavoliniSusanna Ricci
Oct 15, 2008·Infection and Immunity·Christy L VenturaCraig E Rubens
Apr 23, 2004·Infection and Immunity·Francesco IannelliGianni Pozzi
Jan 25, 2005·Infection and Immunity·Ravichandran PalaniappanJames W Lillard
May 24, 2011·Journal of Bacteriology·Robert E TyxAnders P Hakansson
Jan 16, 2007·Journal of Bacteriology·Zhuo Ma, Jing-Ren Zhang
Aug 2, 2008·PloS One·Huaiqing ChenJing-Ren Zhang
Mar 23, 2011·PloS One·Anders P HakanssonCatharina Svanborg
Jun 21, 2005·Emerging Infectious Diseases·Crystal N JohnsonKen B Waites
Oct 14, 2014·Vaccines·Shifeng Wang, Roy Curtiss
Mar 5, 2004·The Lancet Infectious Diseases·D BogaertP W M Hermans
Mar 28, 2008·Future Microbiology·Julie A Preston, David H Dockrell
Sep 7, 2013·The Journal of Infectious Diseases·Sarah BrowallBirgitta Henriques-Normark
May 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alistair J StandishJ C Paton
Jul 13, 2014·International Journal of Pediatric Otorhinolaryngology·Patricia A SchachernSteven K Juhn
Dec 28, 2012·Cellular and Molecular Life Sciences : CMLS·E N MiyajiPaulo Lee Ho
Jul 31, 2013·Critical Reviews in Microbiology·Michelle DarrieuxLuciana Cezar de Cerqueira Leite
Oct 16, 2004·Expert Review of Vaccines·J T Poolman
Apr 26, 2011·Current Opinion in Immunology·Kristin L Moffitt, Richard Malley
Feb 26, 2008·Vaccine·Albert Sabirov, Dennis W Metzger
Feb 28, 2007·Vaccine·Michèle Anne BarocchiRino Rappuoli
Jul 19, 2003·Scandinavian Journal of Immunology·H Jakobsen, I Jonsdottir
Jan 30, 2015·Frontiers in Cellular and Infection Microbiology·Yashuan ChaoAnders P Hakansson
Aug 6, 2003·FEMS Immunology and Medical Microbiology·E Swiatlo, Daphne Ware
Oct 6, 2006·FEMS Microbiology Letters·Lisa R QuinL S McDaniel
Mar 24, 2016·MBio·Lance E KellerL S McDaniel
Jul 2, 2014·Infection and Immunity·Cuong Thach NguyenDong-Kwon Rhee
Mar 1, 2008·Proteomics. Clinical Applications·Jason N ColeMark J Walker
Jun 5, 2007·Expert Review of Vaccines·M L S OliveiraPaulo Lee Ho
Apr 7, 2006·The Journal of Biological Chemistry·Ling LuJing-Ren Zhang
Apr 22, 2015·The Journal of Biological Chemistry·Ulrike BinskerSven Hammerschmidt
Jan 7, 2020·Expert Review of Vaccines·Thiago R ConversoLuciana C C Leite
Sep 19, 2003·The Journal of Biological Chemistry·Ling LuJing-Ren Zhang
Mar 11, 2003·Clinical and Diagnostic Laboratory Immunology·Sandra Romero-SteinerE Ades
Nov 14, 2006·The Journal of Biological Chemistry·Ye ChenPyong Woo Park
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Antoine Dieudonné-VatranBarbara Albiger
Oct 30, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Reshmi MukerjiD E Briles
Jan 28, 2014·PloS One·Effua UsufAndrew Hall
Nov 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ling LuJing-Ren Zhang
Mar 8, 2017·Frontiers in Microbiology·Greiciely O AndreMichelle Darrieux
Dec 25, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Yi YuChangting Liu
Jun 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·S DaveL S McDaniel
May 11, 2019·Microbiology Resource Announcements·R JungesF C Petersen

Related Concepts

Pneumococcal surface protein A
Administration, Intranasal
Metazoa
Bacterial Proteins
Carrier of Disorder
Variolation
Mice, Inbred BALB C
Mice, Inbred CBA
Structure of Choanae
Pneumococcal Infections

Related Feeds

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.