Role of polymerase chain reaction and immunocytochemistry in the cytological assessment of lymphoid proliferations

Journal of Clinical Pathology
L VenkatramanN H Anderson

Abstract

Fine-needle aspiration cytology (FNAC) is used as a screening test to evaluate lymphadenopathy. The combined use of genetic analysis and flow cytometry for immunophenotyping has increased the accuracy of diagnosis and correct categorisation of lymphomas on cytological preparations. To show the utility of immunocytochemistry and polymerase chain reaction (PCR) in the evaluation of cytological preparations of lymph nodes. Fine needle aspirates were obtained from 33 patients (initial presentation, n = 27; recurrence, n = 6). Routine examination was undertaken using immunocytochemistry and DNA PCR to detect clonality and specific translocations. The cytodiagnosis and subclassification of lymphoma was correlated with histological diagnosis in the available follow-up biopsies. 14 patients had a cytological diagnosis of non-Hodgkin's lymphoma (NHL), 4 had suspected NHL, 2 had atypical lymphoid proliferation and 13 had reactive hyperplasia. A World Health Organization (WHO) subtype was suggested in 8 patients. Incorporating the results of immunoglobulin heavy chain (IgH) and T-cell receptor (TCR) gene rearrangements enabled diagnosis of lymphoma in 17 patients, including 5 of the 6 patients suspected to have NHL or an atypical lymphoid...Continue Reading

References

Apr 1, 1997·Cytopathology : Official Journal of the British Society for Clinical Cytology·M D JeffersA F Mutch
Jun 1, 1997·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·A AielloS Pilotti
Jun 25, 1998·Leukemia & Lymphoma·F VianelloF Dazzi
Jul 11, 1998·Journal of Clinical Pathology·C J StewartJ Richmond
Jul 8, 2000·American Journal of Clinical Pathology·T L NicolM J Borowitz
May 16, 2001·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·H Y DongM B Pitman
Jul 2, 2002·Radiology·Nicholas J ScreatonJohn W Grant
Jul 31, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Sean T HehnThomas P Miller
May 27, 2005·Journal of Clinical Pathology·G Kocjan

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Citations

May 7, 2011·The British Journal of Oral & Maxillofacial Surgery·M K HerdP A Brennan
May 24, 2012·Diagnostic Cytopathology·Rachel C Ochs, Adam Bagg
Jul 8, 2009·Diagnostic Cytopathology·Immacolata CozzolinoPio Zeppa
Jan 23, 2015·Archives of Pathology & Laboratory Medicine·John K FrederiksenW Richard Burack
Jul 11, 2018·Cytopathology : Official Journal of the British Society for Clinical Cytology·Helena BarrocaPio Zeppa
Mar 23, 2017·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Michaela SvachovaJan Bouchal
Aug 9, 2019·Experimental and Molecular Pathology·Pei-Hang LeeWan-Ting Huang

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