Role of position 627 of PB2 and the multibasic cleavage site of the hemagglutinin in the virulence of H5N1 avian influenza virus in chickens and ducks.

PloS One
Karel A SchatJohn W Lowenthal

Abstract

Highly pathogenic H5N1 avian influenza viruses have caused major disease outbreaks in domestic and free-living birds with transmission to humans resulting in 59% mortality amongst 564 cases. The mutation of the amino acid at position 627 of the viral polymerase basic-2 protein (PB2) from glutamic acid (E) in avian isolates to lysine (K) in human isolates is frequently found, but it is not known if this change affects the fitness and pathogenicity of the virus in birds. We show here that horizontal transmission of A/Vietnam/1203/2004 H5N1 (VN/1203) virus in chickens and ducks was not affected by the change of K to E at PB2-627. All chickens died between 21 to 48 hours post infection (pi), while 70% of the ducks survived infection. Virus replication was detected in chickens within 12 hours pi and reached peak titers in spleen, lung and brain between 18 to 24 hours for both viruses. Viral antigen in chickens was predominantly in the endothelium, while in ducks it was present in multiple cell types, including neurons, myocardium, skeletal muscle and connective tissues. Virus replicated to a high titer in chicken thrombocytes and caused upregulation of TLR3 and several cell adhesion molecules, which may explain the rapid virus disse...Continue Reading

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Citations

Jun 5, 2013·Virus Research·Gülsah GabrielHans-Dieter Klenk
Mar 15, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Haibo WuNanping Wu
Jan 8, 2013·Comparative Immunology, Microbiology and Infectious Diseases·Hendra WibawaJoanne Meers
Sep 6, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Haibo WuNanping Wu
Dec 19, 2014·Frontiers in Microbiology·Kirsty R ShortThijs Kuiken
Feb 21, 2018·The Journal of General Virology·Leina B SaitoKatharine E Magor
Jul 13, 2018·Influenza and Other Respiratory Viruses·Ye GeHualan Chen
Oct 20, 2018·Avian Diseases·I M Gimeno, K A Schat
Apr 9, 2020·Transboundary and Emerging Diseases·Yulei LiYanbing Li
Jan 11, 2022·Influenza and Other Respiratory Viruses·Supassama ChaiyawongAlongkorn Amonsin

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Datasets Mentioned

BETA
A-MEXP-2133
E-MEXP-3432

Methods Mentioned

BETA
electrophoresis
PCR
serum collection

Software Mentioned

Genowiz
clone manager
NimbleScan
ArrayExpress

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