Role of positively charged residues of the second transmembrane domain in the ion transport activity and conformation of human uncoupling protein-2

Biochemistry
Tuan HoangMasoud Jelokhani-Niaraki

Abstract

Residing at the inner mitochondrial membrane, uncoupling protein-2 (UCP2) mediates proton transport from the intermembrane space (IMS) to the mitochondrial matrix and consequently reduces the rate of ATP synthesis in the mitochondria. The ubiquitous expression of UCP2 in humans can be attributed to the protein's multiple physiological roles in tissues, including its involvement in protective mechanisms against oxidative stress, as well as glucose and lipid metabolisms. Currently, the structural properties and ion transport mechanism of UCP2 and other UCP homologues remain poorly understood. UCP2-mediated proton transport is activated by fatty acids and inhibited by di- and triphosphate purine nucleotides. UCP2 also transports chloride and some other small anions. Identification of key amino acid residues of UCP2 in its ion transport pathway can shed light on the protein's ion transport function. On the basis of our previous studies, the second transmembrane helix segment (TM2) of UCP2 exhibited chloride channel activity. In addition, it was suggested that the positively charged residues on TM2 domains of UCPs 1 and 2 were important for their chloride transport activity. On this basis, to further understand the role of these pos...Continue Reading

References

Aug 1, 1993·Protein Science : a Publication of the Protein Society·N J Greenfield, S E Hitchcock-DeGregori
Feb 2, 1996·The Journal of Biological Chemistry·K D GarlidP Jezek
Jun 1, 1999·FEBS Letters·K S EchtayM Klingenberg
Mar 15, 2000·Journal of Molecular Biology·W P Russ, D M Engelman
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·K S EchtayM Klingenberg
Mar 10, 2001·Biochimica Et Biophysica Acta·M Klingenberg, K S Echtay
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A BakerJ A McCammon
Jan 10, 2002·Nature·Karim S EchtayMartin D Brand
Feb 13, 2002·Current Opinion in Structural Biology·Warren L DeLano
Oct 17, 2003·The Journal of Biological Chemistry·Kathy PfeifferHermann Schägger
Nov 7, 2003·Nature·Eva Pebay-PeyroulaGérard Brandolin
Jun 25, 2004·Nucleic Acids Research·Todd J DolinskyNathan A Baker
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·Stefan KraussBradford B Lowell
Oct 15, 2005·Nature Reviews. Neuroscience·Zane B AndrewsTamas L Horvath
Jan 24, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Valeri BeckElena E Pohl
Apr 21, 2007·Nature Protocols·Karin L Heckman, Larry R Pease
Oct 16, 2007·Free Radical Biology & Medicine·Karim S Echtay
May 31, 2008·Plant & Cell Physiology·Maura Nicoletta LausDonato Pastore
Jul 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yi Wang, Emad Tajkhorshid
Nov 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Alan J RobinsonEdmund R S Kunji
Jan 20, 2009·Bioinformatics·Andrew M WaterhouseGeoffrey J Barton
Aug 1, 2009·Biochemistry·George HarauzJoan M Boggs
Dec 17, 2009·Biochemistry·Marina V IvanovaMasoud Jelokhani-Niaraki
Jan 27, 2010·FEBS Letters·Zuzana Tomaskova, Karol Ondrias
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lars D Renner, Douglas B Weibel
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Jan 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Angelo VozzaGiuseppe Fiermonte
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jonathan J RuprechtEdmund R S Kunji

❮ Previous
Next ❯

Citations

Sep 20, 2016·Oxidative Medicine and Cellular Longevity·Angélica Ruiz-RamírezMohammed El-Hafidi
Jan 21, 2018·Antioxidants & Redox Signaling·Petr JežekMartin Jabůrek
Jul 17, 2015·Bioscience Reports·Tuan HoangMasoud Jelokhani-Niaraki
Aug 10, 2021·The Journal of Physical Chemistry. B·Afshan ArdalanMasoud Jelokhani-Niaraki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.