PMID: 8955294Dec 1, 1996Paper

Role of precursor translocation in coordination of murein and phospholipid synthesis in Escherichia coli

Journal of Bacteriology
K Ehlert, J V Höltje

Abstract

Inhibition of phospholipid synthesis in Escherichia coli by either cerulenin treatment or glycerol starvation of a glycerol-auxotrophic mutant resulted in a concomitant block of murein synthesis. The intracellular pool of cytoplasmic and lipid-linked murein precursors was not affected by an inhibition of phospholipid synthesis, nor was the activity of the penicillin-binding proteins. In addition, a decrease in the activity of the two lipoprotein murein hydrolases, the lytic transglycosylases A and B, could not be demonstrated. The indirect inhibition of murein synthesis by cerulenin resulted in a 68% decrease of trimeric muropeptide structures, proposed to represent the attachment points of newly added murein. Importantly, inhibition of phospholipid synthesis also inhibited O-antigen synthesis with a sensitivity and kinetics similar to those of murein synthesis. It is concluded that the step common for murein and O-antigen synthesis, the translocation of the respective bactoprenolphosphate-linked precursor molecules, is affected by an inhibition of phospholipid synthesis. Consistent with this assumption, it was shown that murein synthesis no longer depends on ongoing phospholipid synthesis in ether-permeabilized cells. We propo...Continue Reading

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Citations

Feb 13, 2008·FEMS Microbiology Reviews·André ZapunMariana G Pinho
May 2, 2014·Frontiers in Microbiology·Catriona Donovan, Marc Bramkamp
Apr 9, 2014·The Journal of Biological Chemistry·Tamimount MohammadiEefjan Breukink
Apr 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Alexander J MeeskeDavid Z Rudner
Jul 5, 2017·Natural Product Reports·Anna MüllerTanja Schneider
Jul 12, 2014·Science·Kevin D Young
Aug 16, 2005·FEMS Microbiology Letters·Beatriz LaraJean van Heijenoort
Dec 18, 2007·FEMS Microbiology Reviews·Ahmed BouhssDominique Mengin-Lecreulx
Mar 22, 2018·F1000Research·Sven van Teeffelen, Lars D Renner
Mar 13, 2002·Journal of Bacteriology·Philippe GérardAndré Zapun
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Dirk-Jan Scheffers, Mariana G Pinho
Dec 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Jean van Heijenoort
May 20, 2020·Nature Reviews. Microbiology·Alexander J F EganWaldemar Vollmer
Dec 5, 2020·Annals of the New York Academy of Sciences·Tobias Dörr
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Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·N Nanninga
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·J V Höltje

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