Role of primers in glucan synthesis by glucosyltransferases from Streptococcus mutans strain OMZ176

Journal of General Microbiology
T KogaS Hamada

Abstract

The effects of isomaltosaccharides of various molecular weights (isomaltose to dextran T2000) on glucan synthesis by a water-soluble glucan-synthesizing glucosyltransferase enzyme (GTase-S) and a water-insoluble glucan-synthesizing enzyme (GTase-I), both from Streptococcus mutans OMZ176, were examined. The activity of GTase-S was not affected by the addition of the isomaltosaccharides, but GTase-I was stimulated increasingly by isomaltosaccharides with degrees of polymerization more than 10. The GTase-I activity first increased and thereafter decreased slightly with increasing amounts of a soluble dextran. Maximal stimulation occurred at concentrations in the range 0.1 to 0.2 mg ml-1, when dextran T10 was used as a primer. The rate of glucan synthesis was highly enhanced by the combined action of GTase-S and GTase-I. The profile of the net activity of GTase-I in the presence of various amounts of GTase-S was similar to that of GTase-I in the presence of increasing amounts of an exogenous dextran. These results collectively suggest that soluble glucan produced by GTase-S from sucrose acts as an intrinsic primer for the glucan synthesis by GTase-I, indicating the contribution of autopriming in glucan synthesis by crude GTase of S...Continue Reading

Citations

Apr 2, 2008·Applied Microbiology and Biotechnology·Anna Maria SwistowskaBernd Hofer
Jul 27, 2001·Bioscience, Biotechnology, and Biochemistry·Noriko Shinozaki-KuwaharaK Fukushima
Jan 1, 1993·DNA Sequence : the Journal of DNA Sequencing and Mapping·S SatoT Katayama
May 26, 2006·Journal of Dental Research·Atsuo Amano
Apr 16, 1999·Oral Microbiology and Immunology·S M ColbyR R Russell
Mar 1, 1984·Journal of Dental Research·S HamadaT Ooshima
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H K Kuramitsu

Related Concepts

Saviosol
Disaccharides
Polycose
Glucosyltransferases
Isomaltose
Streptococcus mutans

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