Role of protein disulfide isomerase and other thiol-reactive proteins in HIV-1 envelope protein-mediated fusion

Virology
Wu Ou, Jonathan Silver

Abstract

Cell-surface protein disulfide isomerase (PDI) has been proposed to promote disulfide bond rearrangements in HIV-1 envelope protein (Env) that accompany Env-mediated fusion. We evaluated the role of PDI in ways that have not been previously tested by downregulating PDI with siRNA and by overexpressing wild-type or variant forms of PDI in transiently and stably transfected cells. These manipulations, as well as treatment with anti-PDI antibodies, had only small effects on infection or cell fusion mediated by NL4-3 or AD8 strains of HIV-1. However, the cell-surface thiol-reactive reagent 5, 5'-dithiobis(2-nitrobenzoic acid) (DTNB) had a much stronger inhibitory effect in our system, suggesting that cell-surface thiol-containing molecules other than PDI, acting alone or in concert, have a greater effect than PDI on HIV-1 Env-mediated fusion. We evaluated one such candidate, thioredoxin, a PDI family member reported to reduce a labile disulfide bond in CD4. We found that the ability of thioredoxin to reduce the disulfide bond in CD4 is enhanced in the presence of HIV-1 Env gp120 and that thioredoxin also reduces disulfide bonds in gp120 directly in the absence of CD4. We discuss the implications of these observations for identifica...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K VuoriK I Kivirikko
Apr 1, 1990·AIDS Research and Human Retroviruses·R L GarlickC S Tomich
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·H J RyserG J DiSciullo
Feb 3, 1999·Annals of the New York Academy of Sciences·C C Wang
Dec 22, 1999·Seminars in Cell & Developmental Biology·R Noiva
Aug 16, 2000·The Journal of Biological Chemistry·S O'NeillN Moran
Feb 15, 2001·Nature Cell Biology·P KellerK Simons
Jun 29, 2002·Nature Immunology·Lisa J MatthiasPhilip J Hogg
Oct 18, 2002·Journal of Cellular Physiology·Carlo TuranoAnna Ferraro
Jul 23, 2003·Biochimica Et Biophysica Acta·Stephen A GalloRobert Blumenthal
Aug 2, 2003·Molecular Membrane Biology·Satinder S RawatAnu Puri
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·John P Moore, Robert W Doms
Sep 19, 2003·AIDS Research and Human Retroviruses·Zhaohao LiaoJames E K Hildreth
Dec 20, 2003·The EMBO Journal·Michael WallinHenrik Garoff
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Kai Simons, Winchil L C Vaz
May 26, 2004·Biochimica Et Biophysica Acta·Bonney Wilkinson, Hiram F Gilbert
Aug 31, 2004·Reviews in Medical Virology·Michael GreenbergLynn Smiley
Jan 13, 2005·Molecular Pharmacology·Rym BarboucheEmmanuel Fenouillet
Sep 27, 2005·Drug Discovery Today·Hugues J-P Ryser, Rudolf Flückiger

❮ Previous
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Citations

Feb 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ping YangNils Stein
Nov 30, 2011·TheScientificWorldJournal·Beatriz S StolfCelio X C Santos
Aug 25, 2006·Journal of Virology·Joanna GilbertThomas Benjamin
Apr 4, 2009·PLoS Pathogens·Stephanie Abromaitis, Richard S Stephens
Mar 6, 2010·The Journal of Biological Chemistry·Marie-Jeanne PapandréouEmmanuel Fenouillet
Oct 15, 2010·The Journal of Biological Chemistry·Iman AzimiPhilip J Hogg
Mar 25, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Avraham AshkenaziYechiel Shai
May 31, 2012·Open Biology·Clive MetcalfeA Neil Barclay
Dec 5, 2012·Retrovirology·Tzanko S StantchevKathleen A Clouse
Jun 15, 2007·Antioxidants & Redox Signaling·Emmanuel FenouilletIan M Jones
Sep 14, 2010·Antioxidants & Redox Signaling·Iman AzimiPhilip J Hogg
Dec 4, 2012·Antioxidants & Redox Signaling·Kristina M Cook, Philip J Hogg
Mar 3, 2007·FEBS Letters·Paul Primakoff, Diana G Myles
Mar 1, 2011·Drug Metabolism Reviews·Kenneth D Tew, Danyelle M Townsend
Jan 11, 2012·The International Journal of Biochemistry & Cell Biology·Kathrin ReiserMathias Lundberg
Dec 17, 2015·Blood·Svenja LippokMaria A Brehm
Nov 29, 2008·The International Journal of Biochemistry & Cell Biology·Joeri AuwerxMathias Lundberg
Jan 16, 2013·Reproduction in Domestic Animals = Zuchthygiene·X J ZhaoW J Xing
Dec 13, 2012·European Journal of Immunology·Monika StegmannA Neil Barclay
Aug 31, 2010·The EMBO Journal·Christian Appenzeller-HerzogLars Ellgaard
Mar 25, 2016·Biochemistry·Gavin Ray OwenNichole Michelle Cerutti
Dec 17, 2014·Acta Microbiologica Et Immunologica Hungarica·Szilvia KanizsaiKároly Nagy
Dec 1, 2012·Acta Microbiologica Et Immunologica Hungarica·Szilvia KanizsaiKároly Nagy
May 14, 2016·World Journal of Virology·Carlos A Guerrero, Orlando Acosta
Jan 22, 2019·Critical Reviews in Microbiology·Signe ChristensenWilhelmina M Huston

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