PMID: 8394097May 1, 1993

Role of protein kinase A in LPS-induced activation of NF-kappa B proteins of a mouse macrophage-like cell line, J774

Cellular Signalling
Masashi Muroi, T Suzuki


The electrophoretic mobility shift assays (EMSA) with the use of the synthetic HIV-1 NF-kappa B motif as a probe, showed that LPS-treatment of J774 cells (a mouse macrophage cell line) leads to the activation of the fast-moving (denoted as B1) and the slow-moving NF-kappa B (denoted as B2). The binding of both B1 and B2 to the NF-kappa B probe was inhibited specifically by either unlabelled NF-kappa B, or competitor probes, but not by unrelated probes. LPS-induced activation of NF-kappa B was inhibited by a protein kinase A (PKA) inhibitor (H-89), but not by a protein kinase C (PKC) inhibitor (H-7). PMA itself failed to activate NF-kappa B and the depletion of PKC did not prevent LPS-induced activation of NF-kappa B. The pre-treatment of J774 cells with dibutyric cAMP, forskolin, prostaglandin E2 or cholera toxin resulted in NF-kappa B activation. Thus, these data suggested a probable involvement of PKA in LPS-induced NF-kappa B activation in macrophages.


Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Cassel, T Pfeuffer
Oct 28, 1988·Science·P A Baeuerle, D Baltimore
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A S Baldwin, P A Sharp
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·S YamamotoJ R McGhee


Aug 29, 1996·European Journal of Pharmacology·C D McWhinneyC K Edwards
Feb 1, 1995·Mutation Research·J RemacleG Rao
May 24, 2005·Biochemical and Biophysical Research Communications·Mifumi Shimomura-ShimizuKen-ichi Tanamoto
Aug 7, 1999·Immunopharmacology·Y J JeonH M Kim
Dec 30, 2009·The Journal of General Physiology·Kazi Mirajul HoqueChung-Ming Tse
May 22, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·L ChiK N Dileepan
Feb 28, 2002·American Journal of Respiratory Cell and Molecular Biology·Youssef OuadrhiriYves Sibille
Jun 26, 2007·Bioscience, Biotechnology, and Biochemistry·Ah-Jin KimJae-Kwan Hwang
Jul 12, 2011·Cellular and Molecular Life Sciences : CMLS·Sarah GerloGuy Haegeman
May 30, 2006·Journal of Insect Physiology·Małgorzata CytryńskaTeresa Jakubowicz
Jun 12, 2012·Experimental Cell Research·Yingting ZhuPeter Lance
Mar 21, 1997·The Journal of Biological Chemistry·K PahanI Singh
Sep 25, 1995·FEBS Letters·G PogliaghiA Bernelli-Zazzera
Jun 7, 2002·The Journal of Biological Chemistry·Vitaliy GavrilyukDouglas L Feinstein
Apr 11, 2014·Clinical and Vaccine Immunology : CVI·Jae Seung YangSeung Hyun Han
Aug 28, 2010·Contrast Media & Molecular Imaging·Jianghua FengRisto Kauppinen
May 19, 2010·Journal of Neurochemistry·Scoty M HearstParminder J S Vig
Jun 6, 1997·The Journal of Biological Chemistry·K C Das, C W White
Jun 16, 2010·Journal of Periodontal Research·K H ZawawiT E Van Dyke
Apr 9, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·M HoriH Karaki
Feb 16, 2017·Journal of Cell Communication and Signaling·Nadine Al Alam, Sawsan Ibrahim Kreydiyyeh
Nov 11, 2018·Cancers·Justyna Struzik, Lidia Szulc-Dąbrowska

Related Concepts

Relb protein, mouse
Cyclic AMP, (R)-Isomer
DNA Sequence
Bucladesine, Barium (1: 1) Salt
NKH 477

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.