PMID: 16641517Apr 28, 2006Paper

Role of protein kinase C, G-protein coupled receptors, and calcium flux during metamorphosis of the sea urchin Strongylocentrotus purpuratus

The Biological Bulletin
G Amador-CanoD Cristino-Jorge

Abstract

Artificial inducers have been used to study signal-transduction pathways involved in metamorphosis of some marine invertebrates. However, the transduction mechanisms for echinoderms have been less explored. In the present study, participation of protein kinase C (PKC), G-protein-coupled receptors (GPCRs), and calcium has been investigated during metamorphosis of the sea urchin Stronglylocentrotus purpuratus. Competent larvae were induced with different drugs that activate (PKC and GP activators, Ca2+ ionophores, and inhibitors of Ca2+ ATPase) or inhibit (PKC, G-protein, and Ca2+ flux inhibitors) metamorphosis. Six of the compounds were effective: the PKC activators TPA and indolactam; the G-protein inhibitors suramin and guanosine; the calcium ionophore A23187, and the calcium ATPase inhibitor thapsigargin. TPA was effective at 0.001 microM; indolactam was effective at 0.001 microM. In the presence of KCl as inducer, the G-protein inhibitor suramin was effective at 10 microM and guanosine at 0.001 microM. In the presence of a bacterial film as inducer, suramin was effective at 50 microM, and guanosine inhibited metamorphosis at 1 microM. A23187 was effective at 5 and 10 microM and thapsigargin at 50 and 100 microM. Our results ...Continue Reading

Citations

Jan 20, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Sandie M Degnan, Bernard M Degnan
Apr 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Brian GaylordMatthew C Ferner
Dec 24, 2018·The Journal of Experimental Biology·Matthew C FernerBrian Gaylord
Sep 10, 2020·Annual Review of Microbiology·Giselle S CavalcantiNicholas J Shikuma
Oct 20, 2011·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Jeanette M Rotchell, Gary K Ostrander
Oct 30, 2020·Marine Pollution Bulletin·Jeric P Da-AnoyCecilia Conaco
Nov 21, 2007·Bioorganic Chemistry·D Thusitha U AbeytungaRobin Polt
Jan 25, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Carlos InfanteManuel Manchado

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.