Role of protein kinase N2 (PKN2) in cigarette smoke-mediated oncogenic transformation of oral cells

Journal of Cell Communication and Signaling
Pavithra RajagopalanAditi Chatterjee

Abstract

Smoking is the leading cause of preventable death worldwide. Though cigarette smoke is an established cause of head and neck cancer (including oral cancer), molecular alterations associated with chronic cigarette smoke exposure are poorly studied. To understand the signaling alterations induced by chronic exposure to cigarette smoke, we developed a cell line model by exposing normal oral keratinocytes to cigarette smoke for a period of 12 months. Chronic exposure to cigarette smoke resulted in increased cellular proliferation and invasive ability of oral keratinocytes. Proteomic and phosphoproteomic analyses showed dysregulation of several proteins involved in cellular movement and cytoskeletal reorganization in smoke exposed cells. We observed overexpression and hyperphosphorylation of protein kinase N2 (PKN2) in smoke exposed cells as well as in a panel of head and neck cancer cell lines established from smokers. Silencing of PKN2 resulted in decreased colony formation, invasion and migration in both smoke exposed cells and head and neck cancer cell lines. Our results indicate that PKN2 plays an important role in oncogenic transformation of oral keratinocytes in response to cigarette smoke. The current study provides evidence...Continue Reading

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Citations

Feb 16, 2019·Omics : a Journal of Integrative Biology·Shankargouda PatilMarco Ferrari
Jan 20, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Shankargouda PatilMarco Ferrari
Jul 20, 2019·Journal of Cell Communication and Signaling·Shankargouda PatilMarco Ferrari
Feb 2, 2021·Biochemical Society Transactions·Georgios SophocleousHelen R Mott
May 2, 2021·Scientific Reports·Shankargouda PatilAditi Chatterjee

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Datasets Mentioned

BETA
PXD007637

Methods Mentioned

BETA
GTPases
transfection

Software Mentioned

Sequest
Proteome Discoverer
Mascot
Ingenuity Pathway Analysis

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