Role of Proteus mirabilis MR/P fimbriae and flagella in adhesion, cytotoxicity and genotoxicity induction in T24 and Vero cells

Pathogens and Disease
Paola ScavonePablo Zunino

Abstract

Proteus mirabilis is frequently associated with complicated urinary tract infections (UTI). It is proposed that several virulence factors are associated with P. mirabilis uropathogenicity. The aim of this work was to elucidate genotoxic and cytotoxic effects mediated by MR/P fimbriae and flagella in eukaryotic cells in vitro. Two cell lines (kidney- and bladder-derived) were infected with a clinical wild-type P. mirabilis strain and an MR/P and a flagellar mutant. We evaluated adhesion, genotoxicity and cytotoxicity by microscopy, comet assay and triple staining technique, respectively. Mutant strains displayed lower adhesion rates than the P. mirabilis wild-type strain and were significantly less effective to induce genotoxic and cytotoxic effects compared to the wild type. We report for the first time that P. mirabilis MR/P fimbriae and flagella mediate genotoxic and cytotoxic effects on eukaryotic cells, at least in in vitro conditions. These results could contribute to design new strategies for the control of UTI.

References

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Citations

Apr 20, 2016·Pathogens and Disease·Paola ScavonePablo Zunino
Jun 15, 2018·Clinical Microbiology Reviews·Amy L HamiltonMark Morrison
Mar 3, 2020·Frontiers in Microbiology·Jinghua CuiJing Yuan
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·María José GonzálezLuciana Robino
Apr 13, 2021·Letters in Applied Microbiology·A P UberM C B Tognim
Feb 10, 2018·EcoSal Plus·Chelsie E ArmbrusterMelanie M Pearson
Aug 28, 2021·International Journal of Molecular Sciences·Paulina StolarekAntoni Różalski

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