Role of Pur alpha in targeting mRNA to sites of translation in hippocampal neuronal dendrites

Journal of Neuroscience Research
Edward M JohnsonJennifer Gordon

Abstract

Using genetic inactivation in the mouse, PURA, encoding Pur alpha, is demonstrated to be essential for developmentally-timed dendrite formation in the cerebellum and hippocampus. Comparison of RNA species bound by Pur alpha prompts the hypothesis that Pur alpha functions with non-coding RNA in transport of certain mRNA molecules to sites of translation in dendrites. Pur alpha binds to human BC200 RNA, implicated in dendritic targeting, and this has homologies to 7SL RNA, implicated in compartmentalized translation. Results using hippocampal rat neurons in situ show that Pur alpha binds to BC1 RNA, implicated in dendritic targeting as a mouse counterpart of BC200, and to mRNA molecules translated in dendrites; Pur alpha is specifically located in dendrites, where it is colocalized with Map2, but not in axons, where it fails to colocalize with Ankyrin G. Pur alpha and Staufen are colocalized at dendritic sites of mRNA translation. Microtubule disruptors inhibit Pur alpha dendritic targeting and allow its mislocalization to axons. Using mouse brain, double-RNA immunoprecipitation places Pur alpha together with Staufen or FMRP on BC1 RNA and specific mRNA species in vivo. These results help define a mechanism by which Pur alpha tar...Continue Reading

References

Mar 25, 1991·Proceedings of the National Academy of Sciences of the United States of America·H TiedgeJ Brosius
May 1, 1990·Brain Research. Brain Research Reviews·R P Tucker
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·W LinkD Kuhl
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·C P KrachmarovE M Johnson
Sep 15, 1998·Trends in Neurosciences·D Goldowitz, K Hamre
Aug 23, 2000·Nucleic Acids Research·G L GalliaK Khalili
Mar 12, 2003·Genes & Development·Yi-Shuian HuangJoel D Richter
Aug 29, 2003·Molecular and Cellular Biology·Boris V SkryabinJürgen Brosius
Mar 19, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Laura N AntarGary J Bassell
Feb 15, 2005·Biochemical and Biophysical Research Communications·Hong LiuEdward M Johnson

❮ Previous
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Citations

Feb 14, 2009·Molecular Neurobiology·Cheryl L Gatto, Kendal Broadie
Apr 16, 2010·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Mauro MaccarroneDiego Centonze
Jan 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anna IacoangeliHenri Tiedge
Apr 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Claudia Bagni
May 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anna IacoangeliHenri Tiedge
Oct 20, 2011·Human Molecular Genetics·Suvi HokkanenJochen Herms
Jan 22, 2008·Biochemistry. Biokhimii︠a︡·J A Makarova, D A Kramerov
Apr 17, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Yuriko KobayashiKaijiro Anzai
Feb 18, 2010·Future Neurology·Jocelyn N Galloway, David L Nelson
Apr 10, 2012·Annual Review of Neuroscience·Asha L BhakarMark F Bear
Aug 3, 2011·The Journal of Cell Biology·Ilham A MuslimovHenri Tiedge
Dec 12, 2012·Trends in Biochemical Sciences·Anna Iacoangeli, Henri Tiedge
Jan 6, 2016·Acta Neuropathologica·J Gavin DaigleUdai Bhan Pandey
Aug 16, 2011·Journal of Neurodevelopmental Disorders·Silvia De Rubeis, Claudia Bagni
Nov 30, 2007·Journal of Neurochemistry·Kerstin DuningJoachim Kremerskothen
May 19, 2006·Current Opinion in Neurobiology·Francesca ZalfaClaudia Bagni
Mar 27, 2009·The Journal of General Virology·H C T GroomA M L Lever
May 27, 2015·Biochemical Society Transactions·James P R SchofieldMark J Coldwell
Oct 16, 2010·Cell Cycle·Rafal KaminskiArmine Darbinyan
Nov 25, 2014·Human Molecular Genetics·Leila K MyrickStephen T Warren
Mar 27, 2013·Journal of Biomolecular Structure & Dynamics·Daniele Di MarinoClaudia Bagni
Jan 27, 2007·Molecular and Cellular Neurosciences·Francesca FerrariClaudia Bagni
Nov 22, 2007·Biological Psychiatry·Diego CentonzeClaudia Bagni
Jan 29, 2017·Cold Spring Harbor Perspectives in Medicine·Tania F Gendron, Leonard Petrucelli
Oct 23, 2015·Cell Death & Disease·M Di SalvioG Cestra
Jan 22, 2014·Journal of Cellular Physiology·Seethalakshmi HariharanArthur Roger Strauch
Sep 29, 2012·Journal of Cellular Physiology·Edward M JohnsonJennifer Gordon
Oct 12, 2013·American Journal of Medical Genetics. Part a·Claudia Bagni, Ben A Oostra
Nov 18, 2006·The Journal of Comparative Neurology·Audra Van WartGary Matthews
Aug 28, 2007·Journal of Cellular Biochemistry·Rafal KaminskiArmine Darbinyan
Nov 1, 2007·Proteomics. Clinical Applications·Wei-Fei DiaoGert Lubec
Jun 20, 2012·The European Journal of Neuroscience·Marco TolinoMichael A Kiebler

❮ Previous
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