Role of Purinergic Receptor P2Y1 in Spatiotemporal Ca2+ Dynamics in Astrocytes

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Eiji ShigetomiSchuichi Koizumi

Abstract

Fine processes of astrocytes enwrap synapses and are well positioned to sense neuronal information via synaptic transmission. In rodents, astrocyte processes sense synaptic transmission via Gq-protein coupled receptors (GqPCR), including the P2Y1 receptor (P2Y1R), to generate Ca2+ signals. Astrocytes display numerous spontaneous microdomain Ca2+ signals; however, it is not clear whether such signals are due to local synaptic transmission and/or in what timeframe astrocytes sense local synaptic transmission. To ask whether GqPCRs mediate microdomain Ca2+ signals, we engineered mice (both sexes) to specifically overexpress P2Y1Rs in astrocytes, and we visualized Ca2+ signals via a genetically encoded Ca2+ indicator, GCaMP6f, in astrocytes from adult mice. Astrocytes overexpressing P2Y1Rs showed significantly larger Ca2+ signals in response to exogenously applied ligand and to repetitive electrical stimulation of axons compared with controls. However, we found no evidence of increased microdomain Ca2+ signals. Instead, Ca2+ waves appeared and propagated to occupy areas that were up to 80-fold larger than microdomain Ca2+ signals. These Ca2+ waves accounted for only 2% of total Ca2+ events, but they were 1.9-fold larger and 2.9-fol...Continue Reading

Citations

Jul 7, 2019·Journal of Neurochemistry·Youichi ShinozakiSchuichi Koizumi
Sep 1, 2019·Current Alzheimer Research·Laura Trujillo-EstradaFrank M LaFerla
Jan 25, 2020·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Julio RiquelmeChristian Bonansco
Feb 11, 2020·British Journal of Pharmacology·Kenneth A JacobsonChrista E Müller
Dec 20, 2018·Science Signaling·Björn-Philipp DiercksAndreas H Guse
Mar 3, 2019·International Journal of Molecular Sciences·Eiji ShigetomiSchuichi Koizumi
Aug 4, 2018·Frontiers in Aging Neuroscience·Patricia KellyBrian J Bacskai
Apr 25, 2020·Physiological Reviews·Christian GiaumeLuc Leybaert
Apr 1, 2020·Neuroscience·Paulo Kofuji, Alfonso Araque
Jul 25, 2021·Respiratory Physiology & Neurobiology·M J OlivaresJ Eugenín

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