Role of RAD51 in the repair of MuDR-induced double-strand breaks in maize (Zea mays L.).

Genetics
Jin LiPatrick S Schnable

Abstract

Rates of Mu transposon insertions and excisions are both high in late somatic cells of maize. In contrast, although high rates of insertions are observed in germinal cells, germinal excisions are recovered only rarely. Plants doubly homozygous for deletion alleles of rad51A1 and rad51A2 do not encode functional RAD51 protein (RAD51-). Approximately 1% of the gametes from RAD51+ plants that carry the MuDR-insertion allele a1-m5216 include at least partial deletions of MuDR and the a1 gene. The structures of these deletions suggest they arise via the repair of MuDR-induced double-strand breaks via nonhomologous end joining. In RAD51- plants these germinal deletions are recovered at rates that are at least 40-fold higher. These rates are not substantially affected by the presence or absence of an a1-containing homolog. Together, these findings indicate that in RAD51+ germinal cells MuDR-induced double-strand breaks (DSBs) are efficiently repaired via RAD51-directed homologous recombination with the sister chromatid. This suggests that RAD51- plants may offer an efficient means to generate deletion alleles for functional genomic studies. Additionally, the high proportion of Mu-active, RAD51- plants that exhibit severe developmental...Continue Reading

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Citations

Dec 15, 2010·International Journal of Biological Sciences·Jun DaiWei Hu
Jun 30, 2010·The Plant Journal : for Cell and Molecular Biology·Wanda M WaterworthChristopher E West
Mar 23, 2015·Plant Reproduction·Arnaud Ronceret, Jean-Philippe Vielle-Calzada
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·L Davis, G R Smith
Feb 20, 2019·International Journal of Molecular Sciences·Fang LiuXiaoyu Li

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