Role of reactive nitrogen species in neuronal cell damage during intestinal schistosomiasis

Cell and Tissue Research
L Van NassauwJ P Timmermans

Abstract

Free radicals are known to be involved in the host reaction during Schistosoma mansoni-induced inflammation in the liver and the intestine. In the present study, the influence of reactive nitrogen species (RNS) on the enteric neurons of infected ileum of mice was investigated. Cryosections and whole-mounts of the ileum of control, and 8- and 15-week-infected mice were processed for immunohistochemical localization of 3-nitrotyrosine, a biomarker of RNS, and of active caspase-3, a key executioner of apoptosis. An antibody directed against protein gene product 9.5 or S100 protein was used as a marker for neurons or enteroglial cells. In infected mice, but not in control animals, 3-nitrotyrosine was detected in parasite eggs and, as revealed by double immunolabelling, in some neuronal and enteroglial cells. Quantitative analysis of whole-mounts showed that the percentage of 3-nitrotyrosine-immunoreactive neurons significantly increased with time in both the submucous and myenteric plexus. Caspase-3 immunoreactivity was predominantly found in parasite eggs in infected mice. Immunoreactive enteric neurons were occasionally observed. The results indicate that inflammation-induced RNS are present in the ileum of S. mansoni-infected mi...Continue Reading

Citations

Nov 20, 2004·Cell and Tissue Research·Huai Zhen Ruan, Geoffrey Burnstock
Sep 3, 2002·International Journal for Parasitology·Joris G De ManPaul A Pelckmans
May 9, 2012·Experimental Diabetes Research·Jerrold PetrofskyHani Al-Nakhli
Jun 10, 2015·Pharmacology, Biochemistry, and Behavior·Zhi XiaoMeng-Jie Sun
Dec 16, 2006·Autonomic Neuroscience : Basic & Clinical·Luc Van NassauwJean-Pierre Timmermans
Mar 25, 2005·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·B Y de WinterP A Pelckmans
Apr 7, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·E Ekblad, A J Bauer
Apr 12, 2003·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·F De JongeJ-P Timmermans
Feb 27, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J Van Op den BoschJ-P Timmermans
Jun 9, 2004·Autonomic Neuroscience : Basic & Clinical·Huai Zhen Ruan, Geoffrey Burnstock
Jan 29, 2011·Neurochemistry International·Shan OuHuai-Zhen Ruan
Dec 10, 2002·Memórias do Instituto Oswaldo Cruz·Allen W CheeverZilton A Andrade
Jul 9, 2014·Nature Reviews. Gastroenterology & Hepatology·Stuart M Brierley, David R Linden
Nov 27, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Andrei SibaevMartin Storr

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