Role of retinoic acid in mouse neural crest cell development in vitro

Developmental Dynamics : an Official Publication of the American Association of Anatomists
K Ito, T Morita

Abstract

This study examines the role of retinoic acid (RA) in mouse neural crest cell development in culture. We have compared the effects of RA on the developmental behavior of mouse neural crest cells from different axial levels, that is the mesencephalic (cranial) and sympathoadrenal (trunk) levels by colony assay using antibodies against cell-type-specific markers. In control cultures in the absence of RA, the efficiency of colony formation by cranial neural crest cells was considerably lower than in colony cultures of trunk neural crest cells. Pulse-labelling experiments using 5-bromo-2'-deoxyuridine (BrdU) showed that the proportion of neural crest cells that incorporated BrdU was significantly smaller in day 5 cultures of cranial neural crest cells in the absence of RA compared to cultures from the trunk level. However, BrdU-incorporation matched the labelling observed in trunk crest cell cultures when RA was added to the culture medium. The efficiency of colony formation and the proportion of BrdU-incorporated cells in trunk crest cell cultures was similar in the presence and absence of RA. The results suggest that in the early stages of in vitro development, RA has a larger impact on proliferation and/or survival of cranial ne...Continue Reading

References

Jan 1, 1991·Development. Supplement·C Tickle
Nov 1, 1991·Developmental Biology·K Ito, M Sieber-Blum
Mar 1, 1991·Teratology·C M Griffith, M J Wiley
Jan 1, 1990·Nouvelle Revue Française D'hématologie·C ChomienneL Degos
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A BaroffioN M Le Douarin
Dec 1, 1989·Neuron·M Bronner-Fraser, S Fraser
Jun 1, 1993·Developmental Biology·K ItoM Sieber-Blum
Apr 1, 1994·Developmental Biology·T A Drysdale, M J Crawford
Jan 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G Morriss-Kay

❮ Previous
Next ❯

Citations

Jul 9, 2009·Cell and Tissue Research·Shinkichi Ishikawa, Kazuo Ito
May 11, 2005·Progress in Neurobiology·Michelle A Lane, Sarah J Bailey
Aug 28, 2003·Developmental Biology·N BhasinA-S LaMantia
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·A B Goodman
Mar 1, 2012·Pigment Cell & Melanoma Research·Yu InoueHirohiko Akamatsu
Apr 1, 2009·Experimental Cell Research·Bruno Costa-SilvaAndréa Gonçalves Trentin
Nov 11, 2005·Progress in Neurobiology·Peter McCaffery, Curtis K Deutsch
May 30, 2016·Cellular and Molecular Neurobiology·Fernanda Rosene MeloAndrea Gonçalves Trentin
Mar 17, 2016·Scientific Reports·Christopher J HindleyJan Pruszak
Dec 18, 2001·Anais Da Academia Brasileira De Ciências·E DupinN Ledouarin
Apr 28, 2006·Journal of Neurochemistry·Olga A AgapovaM Rosario Hernandez
Mar 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y Kubota, K Ito
May 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A Iulianella, D Lohnes
Jul 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mitsunori Ota, Kazuo Ito
Aug 11, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·P NeuvilleG Gabbiani
Jul 12, 2018·Journal of Occupational and Environmental Hygiene·Jonathan SuhlUNKNOWN National Birth Defects Prevention Study
Jul 26, 2006·International Journal of Dermatology·Jamison E Strahan, Sharon Raimer
Oct 8, 2008·Bioscience, Biotechnology, and Biochemistry·Kazuomi SatoMasaru Toriyama
Jul 7, 2011·Current Protocols in Neuroscience·Kazuo Ito, Kyohei Fujita
Apr 16, 1998·Reproductive Toxicology·F ClotmanJ J Picard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Mitsunori Ota, Kazuo Ito
Developmental Dynamics : an Official Publication of the American Association of Anatomists
R M Langille
Developmental Dynamics : an Official Publication of the American Association of Anatomists
Mitsunori Ota, Kazuo Ito
© 2021 Meta ULC. All rights reserved