Role of retrograde trafficking in stress response, host cell interactions, and virulence of Candida albicans

Eukaryotic Cell
Yaoping LiuScott G Filler

Abstract

In Saccharomyces cerevisiae, the vacuolar protein sorting complexes Vps51/52/53/54 and Vps15/30/34/38 are essential for efficient endosome-to-Golgi complex retrograde transport. Here we investigated the function of Vps15 and Vps51, representative members of these complexes, in the stress resistance, host cell interactions, and virulence of Candida albicans. We found that C. albicans vps15Δ/Δ and vps51Δ/Δ mutants had abnormal vacuolar morphology, impaired retrograde protein trafficking, and dramatically increased susceptibility to a variety of stressors. These mutants also had reduced capacity to invade and damage oral epithelial cells in vitro and attenuated virulence in the mouse model of oropharyngeal candidiasis. Proteomic analysis of the cell wall of the vps51Δ/Δ mutant revealed increased levels of the Crh11 and Utr2 transglycosylases, which are targets of the calcineurin signaling pathway. The transcript levels of the calcineurin pathway members CHR11, UTR2, CRZ1, CNA1, and CNA2 were elevated in the vps15Δ/Δ and vps51Δ/Δ mutants. Furthermore, these strains were highly sensitive to the calcineurin-specific inhibitor FK506. Also, deletion of CHR11 and UTR2 further increased the stress susceptibility of these mutants. In cont...Continue Reading

References

Mar 1, 1995·The Journal of Cell Biology·T A Vida, S D Emr
Oct 16, 2001·Antimicrobial Agents and Chemotherapy·Y KamaiS G Filler
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 16, 2002·The EMBO Journal·M Cristina CruzJoseph Heitman
Oct 16, 2002·The Journal of Biological Chemistry·Symeon Siniossoglou, Hugh R B Pelham
Apr 11, 2003·Molecular Biology of the Cell·Elizabeth ConibearTom H Stevens
May 26, 2005·Biochimica Et Biophysica Acta·Katherine Bowers, Tom H Stevens
Aug 31, 2006·Cellular Microbiology·Lisa Y ChiangScott G Filler
Dec 27, 2008·Cell·Ludger Johannes, Vincent Popoff
Aug 25, 2009·Eukaryotic Cell·Hyunsook ParkScott G Filler
Oct 15, 2011·PLoS Pathogens·Yaoping LiuScott G Filler
Mar 10, 2012·Nature Protocols·Norma V Solis, Scott G Filler

❮ Previous
Next ❯

Citations

Jan 2, 2007·PLoS Pathogens·Scott G Filler, Donald C Sheppard
Sep 30, 2016·Nature Communications·Kyung-Tae LeeYong-Sun Bahn
Feb 14, 2017·PLoS Pathogens·Hayet LabbaouiMartine Bassilana
Feb 24, 2018·Frontiers in Cellular and Infection Microbiology·Hagit Bar-YosefDaniel Kornitzer
Feb 11, 2015·Antimicrobial Agents and Chemotherapy·Arturo Luna-TapiaGlen E Palmer
Sep 21, 2016·Antimicrobial Agents and Chemotherapy·Arturo Luna-TapiaGlen E Palmer
Feb 20, 2018·Fungal biology and biotechnology·Zinnat ShahinaTanya E S Dahms
Feb 28, 2020·Journal of Fungi·Christiane RollenhagenSamuel A Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.