Role of Rho-kinase in mediating contraction of chicken embryo femoral arteries.

Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
Bea ZoerEduardo Villamor

Abstract

Rho-kinase-dependent Ca2+ sensitization is an essential process for contraction of mammalian vascular smooth muscle but the information about its effects in non-mammalian vessels is scarce. We aimed to investigate, using the Rho-kinase inhibitor hydroxyfasudil, the potential role of the Rho-kinase pathway of Ca2+ sensitization in depolarization- and agonist-mediated contraction of chicken embryo (at day 19 of the 21 days of incubation) femoral arteries. Contraction elicited by KCl (125 mM) comprised two phases (phasic and tonic contraction), both of which were abolished in the absence of extracellular Ca2+. Hydroxyfasudil (10 microM) left the initial phasic component nearly intact but abolished the tonic component. Hydroxyfasudil also induced a marked impairment of the contractions elicited by phenylephrine (PE), the thromboxane A2 mimetic U46619, and endothelin-1. In contrast, inhibition of protein kinase C (PKC) by chelerythrine did not affect KCl- or PE-induced contractions, indicating lack of participation of PKC-mediated Ca2+ sensitization. Incubation under chronic hypoxia (15% O2 from day 0) impaired embryonic growth but did not significantly affect hydroxyfasudil-mediated relaxation. In summary, our findings are indicati...Continue Reading

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Citations

Jun 1, 2011·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Rob M Moonen, Eduardo Villamor
Dec 12, 2012·Scandinavian Journal of Gastroenterology·Ming-Che LeeShih-Che Huang
Jun 30, 2015·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Riazudin MohammedEduardo Villamor
Jul 29, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Isa LindgrenJordi Altimiras
Jan 16, 2016·Journal of Applied Physiology·Aleksandra MazurFrançois Guerrero

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