Role of ribonucleic acid synthesis in conjugational transfer of chromosomal and plasmid deoxyribonucleic acids.

Journal of Bacteriology
L J Maturin, R Curtiss

Abstract

A strain of Escherichia coli K-12 containing mutations that allow for the experimental control of RNA and DNA syntheses was constructed to investigate the role that RNA synthesis plays in conjugational DNA transfer when DNA replication is inhibited. The mutations possessed by this strain and its donor derivatives include: (i) thyA, which blocks synthesis of dTMP, causing a requirement for thymine; (ii) deoC, which blocks breakdown of deoxyribose 5-phosphate, permitting growth with low levels of thymine; (iii) pyrF, which blocks synthesis of UMP from OMP, imposing a requirement for uridine; (iv) cdd and pyrG, which block the deamination of cytidine to uridine and the synthesis of CTP from UTP, respectively, causing a requirement for cytidine; (v) codA and codB, which block the deamination of cytosine to uracil and cytosine transport, respectively, preventing the substitution of cytosine for cytidine; and (vi) dnaB, which blocks vegetative but not conjugational DNA replication at 42 degrees C. DNA synthesis can be blocked in the donor strains by the addition of excess uridine when exogenous thymine is not present. We found that RNA synthesis can also be blocked by addition of excess uridine when exogenous cytidine is not present....Continue Reading

References

Dec 15, 1977·Nature·N KennedyP Herrlich
Mar 1, 1978·Journal of Bacteriology·M AchtmanA J Clark
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E LankaH Schuster
Oct 1, 1971·Journal of Bacteriology·D VapnekW D Rupp
Mar 23, 1968·Nature·G G Meynell, A M Lawn
Jun 1, 1968·Journal of Bacteriology·J Neuhard, J Ingraham
Sep 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·A CohenH I Adler
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·W Gilbert, D Dressler
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·K W Fisher, M B Fisher
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·A CohenH I Adler
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·W D Rupp, G Ihler
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·M Ohki, J Tomizawa
Jan 1, 1969·Annual Review of Microbiology·R Curtiss
Nov 1, 1969·Journal of Bacteriology·R CurtissD R Stallions
Sep 1, 1970·Bacteriological Reviews·G A O'Donovan, J Neuhard
Oct 1, 1965·Journal of Bacteriology·D Freifelder
Mar 1, 1980·Microbiological Reviews·B J Bachmann, K B Low
Feb 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·H I AdlerA A Hardigree

❮ Previous
Next ❯

Citations

Dec 1, 1981·Applied Biochemistry and Biotechnology·A RembaumH L Heyneker
Mar 1, 1984·Microbiological Reviews·N Willetts, B Wilkins

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.