PMID: 6163463Jan 29, 1981Paper

Role of ribosome recycling in uptake of dihydrostreptomycin by sensitive and resistant Escherichia coli

Biochimica Et Biophysica Acta
C HurwitzC L Rosano

Abstract

Exposure of streptomycin-resistant cells to puromycin results in uptake of dihydrostreptomycin comparable to that found with streptomycin-sensitive cells. This finding indicates that the enhanced phase of uptake, previously reported only in sensitive cells, may result from an increase in internal binding sites, presumably run-off ribosomes. The increased uptake of dihydrostreptomycin resulting from exposure to puromycin is greatest in both sensitive and resistant cells at concentrations below 100 microgram/ml. At 100 microgram/ml, exposure to puromycin in vivo results in significant, but not complete, polysome degradation and inhibition of protein synthesis. At 500 microgram/ml, where polysome degradation is complete in less than 2 min and where growth and protein synthesis are inhibited more than 90%, uptake of dihydrostreptomycin by both sensitive and resistant cells is inhibited. Puromycin has no effect on binding of dihydrostreptomycin to 70-S monosomes, as measured by equilibrium dialysis. The increased uptake of dihydrostreptomycin by resistant cells resulting from exposure to puromycin has no effect on viability. Addition of N-ethylmaleimide immediately and completely inhibits the puromycin-induced uptake of dihydrostrep...Continue Reading

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Citations

Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B D DavisP C Tai
Oct 1, 1984·Antimicrobial Agents and Chemotherapy·L J MandelM H Miller
Apr 1, 1990·Antimicrobial Agents and Chemotherapy·M A Wyka, A C St John
Nov 1, 1982·Antimicrobial Agents and Chemotherapy·N E AllenH A Kirst
Sep 14, 2010·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Maria S Ramirez, Marcelo E Tolmasky
Apr 7, 2017·Molecular Microbiology·Marion HerisseBenjamin Ezraty
Dec 20, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria S Ramirez, Marcelo E Tolmasky
Aug 24, 2018·Antibiotics·Frédéric BarrasBenjamin Ezraty
Oct 23, 2019·Journal of Biomolecular Structure & Dynamics·Dhamodharan PrabhuJeyaraman Jeyakanthan
Aug 22, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·E Azucena, S Mobashery
Dec 1, 1987·Microbiological Reviews·H W TaberA S Arrow
Nov 1, 2012·ACS Chemical Biology·Bernd Becker, Matthew A Cooper

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