PMID: 6163994Apr 30, 1981Paper

Role of RNA transcripts in replication incompatibility and copy number control in antibiotic resistance plasmid derivatives

Nature
J RosenE Ohtsubo

Abstract

The genes required for autonomous replication and incompatibility in the antibiotic resistance plasmids R100 and R1 have been located within a 2.5-kilobase region of the 90-kilobase genome, within which the incompatibility gene occupies a 1.3-kilobase region excluding the replication origin. We now report that three RNA species are synthesized in vitro from the 2.5-kilobase region, which R100 and R1 have in common. One, a long RNA molecule which is transcribed in the direction of DNA replication, probably acts as a messenger or a protein required for plasmid replication. The second RNA species, only 91 nucleotides long, is transcribed in the opposite direction, from a region of the DNA entirely contained within the first and known to specify incompatibility and copy control functions. The third RNA species, 150 bases long, is transcribed from a region including the replication origin; it may be a primer of DNA synthesis or, in conjunction with the second of the three RNA species, an influence in the control of replication.

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Citations

Jan 1, 1982·Molecular & General Genetics : MGG·J Light, S Molin
Jan 1, 1985·Molecular & General Genetics : MGG·J LightS Molin
Jan 1, 1983·Molecular & General Genetics : MGG·J Light, S Baumberg
Jan 1, 1989·Molecular & General Genetics : MGG·H YasuedaT Itoh
Oct 1, 1986·Molecular & General Genetics : MGG·K A ArmstrongE Ohtsubo
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P StougaardK Nordström
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H DanbaraK N Timmis
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H MasaiK Arai
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T MizunoM Inouye
Feb 1, 1986·Journal of Biomolecular Structure & Dynamics·V BrendelE N Trifonov
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
May 25, 1988·Nucleic Acids Research·M J AlldayB E Griffin
May 10, 2005·Immunology and Cell Biology·Dipanjan Chowdhury, Carl D Novina
Sep 30, 1991·Biochemical and Biophysical Research Communications·V VollochS Rits
Nov 26, 1981·Nature·H Lother, W Messer
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Oct 1, 1984·Journal of Bacteriology·D D WombleR H Rownd
Dec 1, 1987·Journal of Bacteriology·X N DongR H Rownd

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