Role of RpoS in stress resistance, quorum sensing and spoilage potential of Pseudomonas fluorescens

International Journal of Food Microbiology
Xiaoxiang LiuAihua Sun

Abstract

Microorganism activities are considered the main cause of most food spoilage, leading to great economic losses. Pseudomonas fluorescens is a Gram-negative bacterium that is widely found in food and has high spoilage activity. RpoS is considered an important global regulator involved in stress survival and virulence in many pathogens. Thus, it is very possible that RpoS plays an important role in spoilage regulation in P. fluorescens. In this study an in-frame deletion mutation of rpoS was constructed to explore its function in P. fluorescens. The results showed that RpoS positively regulated the resistance of P. fluorescens to H2O2, heat, ethanol and crystal violet, negatively regulated the resistance to acetic acid, and had no effect on the resistance to NaCl. Further studies indicated that acylated homoserine lactone (AHL) production and the transcription levels of five AHL-related genes were significantly decreased in the rpoS mutant compared with those in the wild-type strain. Interestingly, the two homologous genes coding for AHL synthases contained RpoS-dependent -10 elements, suggesting that AHL quorum sensing is directly regulated by RpoS. RpoS also contributed to the spoilage activities of P. fluorescens by regulating ...Continue Reading

Citations

Oct 4, 2019·Environmental Microbiology·Matthew McIntoshGabriele Klug
Oct 31, 2020·Letters in Applied Microbiology·Caili ZhangYanlong Liu
Sep 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Nirmani N WickramasingheGary A Dykes

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