PMID: 8595982Aug 1, 1995Paper

Role of scalar protons in metabolic energy generation in lactic acid bacteria

Journal of Bioenergetics and Biomembranes
J S LolkemaW N Konings

Abstract

Lactic acid bacteria are able to generate a protonmotive force across the cytoplasmic membrane by various metabolic conversions without involvement of substrate level phosphorylation or proton pump activity. Weak acids like malate and citrate are taken up in an electrogenic process in which net negative charge is translocated into the cell thereby generating a membrane potential. The uptake is either an exchange process with a metabolic end-product (precursor/ product exchange) or a uniporter mechanism. Subsequent metabolism of the internalized substrate drives uptake and results in the generation of a pH gradient due to the consumption of scalar protons. The generation of the membrane potential and the pH gradient involve separate steps in the pathway. Here it is shown that they are nevertheless coupled. Analysis of the pH gradient that is formed during malolactic fermentation and citrate fermentation shows that a pH gradient, inside alkaline, is formed only when the uptake system forms a membrane potential, inside negative. These secondary metabolic energy generating systems form a pmf that consists of both a membrane potential and a pH gradient, just like primary proton pumps do. It is concluded that the generation of a pH g...Continue Reading

References

Jan 1, 1991·European Journal of Biochemistry·M E Van der RestW N Konings
Dec 1, 1985·Journal of Bacteriology·N Ishiguro, G Sato
Oct 1, 1994·European Journal of Biochemistry·M SalemaW N Konings
Sep 1, 1993·FEMS Microbiology Reviews·B Poolman

❮ Previous
Next ❯

Citations

Apr 5, 2007·Archives of Microbiology·Yoann AugagneurJean Guzzo
May 28, 2013·Applied and Environmental Microbiology·Pablo MorteraJuke S Lolkema
Dec 25, 2007·Applied and Environmental Microbiology·Claudia SánchezHelena Santos
May 9, 2012·Journal of Bacteriology·Agata M Pudlik, Juke S Lolkema
May 15, 2007·Journal of Bacteriology·R J W BrooijmansJ Hugenholtz
Nov 28, 2007·Journal of Bacteriology·Gloria HerrmannWolfgang Buckel
Jul 21, 2005·Journal of Bacteriology·Mauricio G MartinPaloma López
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Iwona Sobczak, Juke S Lolkema
Aug 8, 2014·International Journal of Food Microbiology·Federico A ZuljanChristian Magni
Sep 20, 2000·The Journal of Biological Chemistry·M Bandell, J S Lolkema
Apr 1, 1996·Journal of Bacteriology·C Marty-TeyssetW N Konings

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.