Role of scavenger receptors in peptide-based delivery of plasmid DNA across a blood-brain barrier model

International Journal of Pharmaceutics
Artita SrimaneeÜlo Langel

Abstract

Receptor-mediated transcytosis remains a major route for drug delivery across the blood-brain barrier (BBB). PepFect 32 (PF32), a peptide-based vector modified with targeting ligand (Angiopep-2) binding to low-density lipoprotein receptor-related protein-1 (LRP-1), was previously found to be a promising vector for plasmid delivery across an in vitro model of the BBB. Cellular uptake of PF32/plasmid DNA (pDNA) complexes was speculated the internalization via LRP-1 receptor. In this study, we prove that PF32/pDNA nanocomplexes are not only transported into brain endothelial cells via LRP-1 receptor-mediated endocytosis, but also via scavenger receptor class A and B (SCARA3, SCARA5, and SR-BI)-mediated endocytosis. SCARA3, SCARA5, and SR-BI are found to be expressed in the brain endothelial cells. Inhibition of these receptors leads to a reduction of the transfection. In conclusion, this study shows that scavenger receptors also play an essential role in the cellular uptake of the PF32/pDNA nanocomplexes.

References

May 16, 1998·Biochemical and Biophysical Research Communications·M NakamuraM Hashida
Nov 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Thomas J F NielandTomas Kirchhausen
Jan 18, 2003·Vascular Pharmacology·J M Scherrmann
Apr 28, 2004·Advanced Drug Delivery Reviews·Nancy S Chung, Kishor M Wasan
Sep 12, 2006·Annual Review of Pharmacology and Toxicology·A G de Boer, P J Gaillard
Jan 3, 2007·Drug Discovery Today·William M Pardridge
Jun 8, 2007·Pharmaceutical Research·William M Pardridge
Jun 29, 2007·Clinical Pharmacokinetics·Albertus G de Boer, Pieter J Gaillard
Jul 11, 2007·Pharmaceutical Research·Angela R Jones, Eric V Shusta
Sep 23, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Karsten UlbrichJörg Kreuter
Mar 31, 2009·Expert Opinion on Drug Delivery·J RipA G de Boer
Apr 28, 2009·Progress in Neurobiology·Ivona BrasnjevicUNKNOWN European NanoBioPharmaceutics Research Initiative
Apr 20, 2010·Osteoarthritis and Cartilage·M Vallières, P du Souich
Oct 29, 2010·International Journal of Hypertension·Sam L StephenSreenivasan Ponnambalam
Dec 6, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kariem EzzatUlo Langel
Mar 23, 2012·Immunobiology·Julia KzhyshkowskaSiamon Gordon
Jun 12, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Gemma ManichJordi Vilaplana
Jul 11, 2013·International Journal of Cell Biology·Guangqing Xiao, Liang-Shang Gan

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Citations

Oct 31, 2016·Advanced Drug Delivery Reviews·Taavi LehtoSamir El Andaloussi
Jan 20, 2017·CNS Drugs·Mayur M Patel, Bhoomika M Patel
May 16, 2017·Journal of Pharmaceutical Sciences·Mie Kristensen, Birger Brodin
Apr 3, 2018·Biological & Pharmaceutical Bulletin·Noriyasu KameiMariko Takeda-Morishita
Feb 13, 2020·Pharmacological Reports : PR·Nuno ValeTiago Fleming Outeiro
Dec 15, 2020·American Journal of Alzheimer's Disease and Other Dementias·Joseph S D'Arrigo
Sep 24, 2019·Biomaterials·Jinbing XieXiaoyuan Chen
May 1, 2021·Pharmaceutics·Abiodun Ayo, Pirjo Laakkonen

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