Role of secretory protease inhibitor SPINK3 in mouse uterus during early pregnancy

Cell and Tissue Research
Wen ChenJing-Pian Peng

Abstract

Successful embryo implantation depends on intricate epithelial-stromal cross-talk. However, molecular modulators involved in this cellular communication remain poorly elucidated. Using multiple approaches, we have investigated the spatiotemporal expression and regulation of serine protease inhibitor Kazal type 3 (SPINK3) in mouse uterus during the estrous cycle and early pregnancy. In cycling mice, both SPINK3 mRNA and protein are only expressed during proestrus. In the pregnant mouse, the expression levels of both SPINK3 mRNA and protein increase on days 5-8 and then decline. Spink3 mRNA is expressed exclusively in the uterine glandular epithelium, whereas SPINK3 protein is localized on the surface of both luminal and glandular epithelium and in the decidua. Moreover, SPINK3 in the decidua has been observed in the primary decidual zone on day 6 and the secondary decidual zone on days 7-8; this is tightly associated with the progression of decidualization. SPINK3 has also been found in decidual cells of the artificially decidualized uterine horn but not control horn, whereas Spink3 mRNA localizes in the glands of both horns. The expression of endometrial Spink3 is not regulated by the blastocyst according to its expression patt...Continue Reading

References

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Citations

Dec 3, 2016·Scientific Reports·Andrew M KelleherThomas E Spencer
Feb 15, 2013·Biology of Reproduction·Justyna Filant, Thomas E Spencer
Sep 13, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Justyna FilantThomas E Spencer
Aug 27, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mizuki SoneKatsuhiko Nishimori
May 11, 2019·Endocrine Reviews·Andrew M KelleherThomas E Spencer
Jan 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Andrew M KelleherThomas E Spencer

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