PMID: 6968782Dec 1, 1980Paper

Role of self carriers in the immune response and tolerance. VI. Induction and reversal of TNP-modified self unresponsiveness in vitro

The Journal of Immunology : Official Journal of the American Association of Immunologists
J T Li, D W Scott

Abstract

The involvement of T-suppressor cells in hapten-modified self unresponsiveness induced in vivo is well established. However, TNP-modified syngeneic spleen cells (TNP-SC) can block the anti-TNP PFC response of normal or nude spleen cells to TNP-immunogen in vitro, an effect that is apparently T cell-independent. In this study, we have used a 2-stage culture system in which both the induction of tolerance by irradiated syngeneic TNP-SC and the subsequent challenge with TNP-immunogen take place in vitro to dissect the cellular events that lead to unresponsiveness. Our results show that not only are spleen cells precultured with TNP-SC tolerant to challenge, but that these cells are able to specifically suppress the response of freshly prepared normal spleen cells to the T-independent antigens TNP-POL or TNP-LPS. Irradiation of the precultured cells abrogates the suppressor activity and rules out hapten carryover as the mechanism of suppression. Suppressor cells were able to inhibit the response of anti-Ba theta and C-treated spleen cells, suggesting that suppressor function is directed toward the B cell and/or accessory cells. Anti-Ba theta and C treatment of spleen cells before preculture with TNP-SC prevented the induction of bo...Continue Reading

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