Role of serine phosphorylation of Stat5a in prolactin-stimulated beta-casein gene expression

Molecular and Cellular Endocrinology
H YamashitaH Rui

Abstract

Milk production remains suppressed in mammals during late pregnancy despite high levels of lactogenic polypeptide hormones. At parturition, associated with a precipitous fall in circulating progesterone, rising glucocorticoid levels synergize with prolactin to initiate copious milk production. This synergy is mediated at least in part through the coordinated activation of glucocorticoid receptors and transcription factor Stat5, particularly Stat5a. Here we show that two proline-juxtaposed serine residues within the transactivation domain of Stat5a are phosphorylated in the mammary gland during late gestation and lactation, and that these phosphorylation sites inhibit the transcriptional activity of Stat5a in the absence of glucocorticoid receptor costimulation. Specifically, transfection assays revealed that phosphorylation of residues S725 and S779 of Stat5a cooperatively suppressed prolactin-stimulated transcription from the beta-casein promoter in both COS-7 kidney and MCF-7 mammary cells. This suppression was associated with shortened duration and reduced amplitude of nuclear DNA binding activity of wild type Stat5a relative to that of the serine phosphorylation-defective Stat5 mutant. However, costimulation of glucocortico...Continue Reading

References

Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·X LiuL Hennighausen
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·J F BrombergJ E Darnell
Jan 15, 1997·Genes & Development·X LiuL Hennighausen
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·G B UdyH W Davey
Jan 5, 1999·The Journal of Biological Chemistry·E N FishL C Platanias
Jul 31, 1999·The Journal of Biological Chemistry·A V KazanskyJ M Rosen
Aug 24, 1999·Cell·J F BrombergJ E Darnell
Sep 10, 1999·The Journal of Biological Chemistry·C LeeC Schindler
Apr 1, 2000·Cytokine & Growth Factor Reviews·P M GrimleyH Rui

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Citations

Dec 3, 2002·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Hiroko Yamashita, Hirotaka Iwase
Jan 31, 2012·Analytical and Bioanalytical Chemistry·Maguy Abi JaoudéLaurence Bois
Apr 25, 2003·Biochemical and Biophysical Research Communications·Franklyn F Bolander
Nov 20, 2009·The Journal of Biological Chemistry·Jeremy A RossRobert A Kirken
Jan 17, 2003·Molecular Biology of the Cell·Salvador Aznar BenitahJuan Carlos Lacal
Dec 31, 2015·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Aradhana Rani, John J Murphy
Sep 8, 2007·Biochemical and Biophysical Research Communications·Amanda M Weaver, Corinne M Silva
Nov 25, 2004·Cytokine & Growth Factor Reviews·Kirsi Paukku, Olli Silvennoinen
Feb 25, 2005·Vitamins and Hormones·Claudia M LitterstEdith Pfitzner
Apr 28, 2004·Biochemical and Biophysical Research Communications·Jaya NautiyalMalini Laloraya
Aug 3, 2006·Journal of Cellular Physiology·Itamar Barash
May 3, 2005·The Journal of Biological Chemistry·Diane E ClarkFrank E Jones
May 10, 2019·Genome Génome / Conseil National De Recherches Canada·Periyasamy VijayakumarRamalingam Suresh
Oct 22, 2014·Oncotarget·Angelika BergerRichard Moriggl
Aug 12, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Thai H TranHallgeir Rui
Apr 13, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David A GewirtzStephen T Sawyer
Nov 17, 2009·The International Journal of Biochemistry & Cell Biology·Zhiyong LiaoMarja T Nevalainen
Oct 29, 2004·The American Journal of Pathology·Charles V Clevenger

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