PMID: 2509423Nov 1, 1989Paper

Role of sigma H in expression of the fumarase gene (citG) in vegetative cells of Bacillus subtilis 168

Journal of Bacteriology
V PriceA Moir

Abstract

The fumarase gene (citG) of Bacillus subtilis is transcribed from two promoter regions, citGp1 and citGp2 (P1 and P2); the P2 promoter is used by the E sigma H form of RNA polymerase. In order to study the role of P1 and P2 in citG expression, the promoter region and various deletion derivatives that effectively separate P1 and P2 were fused to the Escherichia coli beta-galactosidase gene (lacZ) and introduced into the chromosome in single copy at the amyE locus. P1 functioned to provide a relatively low and stable basal level of fumarase activity throughout growth. In contrast, P2 activity was found to vary over at least a 50-fold range and was responsible for regulating fumarase activity during growth and sporulation in a rich medium and in response to changes in carbon source. To further investigate the role of sigma H in fumarase regulation, citGp2-lacZ fusions were introduced into a strain in which the expression of the chromosomal spoOH gene was under the control of the isopropylthiogalactopyranoside-inducible spac promoter. Induction of pspac did not lead to P2 induction, suggesting that citG expression is not regulated at the level of spoOH transcription.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 1, 1986·Journal of Bacteriology·M LewandoskiI Smith
Feb 1, 1989·Journal of Bacteriology·J J WuP J Piggot
Nov 1, 1989·Journal of Bacteriology·K M TattiC P Moran
Oct 1, 1986·Molecular & General Genetics : MGG·S YamashitaH Saito
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H L Carter, C P Moran
Jul 1, 1987·Journal of Bacteriology·M AlbanoD Dubnau
Mar 1, 1988·Journal of Bacteriology·N K GaurI Smith
Mar 1, 1988·Molecular & General Genetics : MGG·I M FeaversA Moir
Jan 11, 1985·Nucleic Acids Research·J S Miles, J R Guest
Apr 1, 1968·Journal of Bacteriology·P Fortnagel, E Freese
Jun 1, 1981·Analytical Biochemistry·D S Holmes, M Quigley
Jun 1, 1983·Journal of Bacteriology·F A FerrariJ A Hoch
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Feb 1, 1984·Journal of Bacteriology·J WeirI Smith
Sep 1, 1981·Plasmid·S B Primrose, S D Ehrlich
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen

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Citations

Feb 25, 2017·PloS One·Luz Adriana Vega-CabreraLiliana Pardo-López
Dec 16, 1998·Journal of Bacteriology·K DrzewieckiM Hecker
Aug 10, 2002·Journal of Bacteriology·Robert A BrittonAlan D Grossman
Jan 25, 2002·Journal of Bacteriology·Jörg SieversJeff Errington
Mar 1, 1995·Microbiological Reviews·W G Haldenwang
Nov 1, 1989·Journal of Bacteriology·K M TattiC P Moran
Aug 1, 1994·Journal of Bacteriology·S Jin, A L Sonenshein
Sep 1, 1990·Journal of Bacteriology·U BaiI Smith

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