Role of SIRT1-mediated mitochondrial and Akt pathways in glioblastoma cell death induced by Cotinus coggygria flavonoid nanoliposomes

International Journal of Nanomedicine
Gang WangJing Wang

Abstract

Flavonoids, the major polyphenol components in Cotinus coggygria (CC), have been found to show an anticancer effect in our previous study; however, the exact mechanisms of inducing human glioblastoma (GBM) cell death remain to be resolved. In this study, a novel polyvinylpyrrolidone K-30/sodium dodecyl sulfate and polyethyleneglycol-coated liposome loaded with CC flavonoids (CCFs) was developed to enhance solubility and the antibrain tumor effect, and the molecular mechanism regarding how CCF nanoliposomes (CCF-NLs) induce apoptotic cell death in vitro was investigated. DBTRG-05MG GBM cell lines treated with CCF-NLs showed potential antiproliferative effects. Regarding the underlying mechanisms of inducing apoptosis in DBTRG-05MG GBM cells, CCF-NLs were shown to downregulate the expression of antiapoptotic B-cell lymphoma/leukemia 2 (Bcl-2), an apoptosis-related protein family member, but the expression of proapoptotic Bcl-2-associated X protein was enhanced compared with that in controls. CCF-NLs also inhibited the activity of caspase-3 and -9, which is the initiator caspase of the extrinsic and intrinsic apoptotic pathways. Blockade of caspase activation consistently induced apoptosis and inhibited growth in CCF-NL-treated DB...Continue Reading

Citations

Sep 9, 2016·Expert Opinion on Drug Delivery·Carmelo PugliaGiovanni Puglisi
Feb 6, 2017·Drug Delivery·Orazio VittorioGiuseppe Cirillo
Feb 28, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Xuezhi ZhangJun Guan
Sep 14, 2017·ACS Nano·Sung Hun KangYong-Kyu Lee

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Methods Mentioned

BETA
ELISA
protein assay
flow cytometry
electrophoresis
transfection
transmission electron microscopy
acetylation

Software Mentioned

SPSS
Pro
Statistical Program for Social Sciences
Image

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