Role of spacer length in interaction between novel gemini imidazolium surfactants and Rhizopus oryzae lipase

International Journal of Biological Macromolecules
Sunita AdakRintu Banerjee

Abstract

An insight into the effects of new ionic liquid-type gemini imidazolium cationic surfactants on the structure and function of the lipases is of prime importance for their potential application. Changes in the activity, stability and structure of Rhizopus oryzae lipase in the presence of novel gemini surfactants, [C16-3-C16im]Br2 and [C16-12-C16im]Br2 were probed in the present study. Surfactant with shorter spacer length, [C16-3-C16im]Br2 was found to be better in improving the hydrolytic activity and thermal stability of the lipase. For both the surfactants, activation was concentration dependent. CD spectroscopy results showed a decrease in α-helix and an increase in β-sheet content in the presence of these surfactants. A higher structural change observed in presence of [C16-12-C16im]Br2 correlated with lower enzyme activity. Isothermal titration calorimetric studies showed the binding to be spontaneous in nature based on sequential two site binding model. The forces involved in binding were found to differ for the two surfactants proving that the spacer length is an important factor which governs the interaction. These surfactants could be used as promising components both in enzyme modification and media engineering for att...Continue Reading

References

Jan 1, 1986·Methods in Enzymology·J T YangH M Martinez
Dec 2, 2000·Biochimie·C M Carvalho, J M Cabral
Mar 22, 2002·Journal of Virology·Fabiana A CarneiroAndrea T Da Poian
Jul 21, 2006·The Journal of Physical Chemistry. B·Paula V MessinaFélix Sarmiento
Aug 3, 2006·Journal of Agricultural and Food Chemistry·Hsin-Ju HsiehWen-Teng Wu
Aug 26, 2006·Biochimica Et Biophysica Acta·Ahmed AloulouFrédéric Carrière
Sep 12, 2006·Protein Science : a Publication of the Protein Society·Laura H LucasC Russell Middaugh
Sep 21, 2007·Bioprocess and Biosystems Engineering·Annapurna KumariRintu Banerjee
Dec 12, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Kell K AndersenDaniel E Otzen
Mar 1, 2008·Colloids and Surfaces. B, Biointerfaces·Run-Chao LuJin-Xin Xiao
Jul 29, 2008·Journal of Colloid and Interface Science·Mingqi AoYan Bai
Aug 6, 2008·Journal of Agricultural and Food Chemistry·Asghar Taheri-KafraniThomas Haertlé
Aug 12, 2008·Advances in Colloid and Interface Science·P ReisR Miller
Jun 10, 2009·Colloids and Surfaces. B, Biointerfaces·Ali Asghar RastegariAsghar Taheri-Kafrani
Feb 11, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Sisir DebnathPrasanta Kumar Das
Feb 17, 2010·Biotechnology Advances·Maria ZoumpaniotiAristotelis Xenakis
Jan 15, 2011·Pharmaceutical Research·Vincent DelormeJean-François Cavalier
Mar 15, 2011·Biochimica Et Biophysica Acta·Daniel Otzen
Mar 29, 2011·Biochimica Et Biophysica Acta·Jon G HanstedDaniel E Otzen
Feb 4, 2012·International Journal of Biological Macromolecules·Razieh AmiriIraj Mohammadpoor-Baltork

❮ Previous
Next ❯

Citations

Jul 27, 2017·Chemical Reviews·Toshiyuki Itoh
Nov 9, 2019·International Journal of Molecular Sciences·Lucia Ya ZakharovaEliana B Souto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.